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Confocal Time Lapse Imaging as an Efficient Method for the Cytocompatibility Evaluation of Dental Composites

Confocal Time Lapse Imaging as an Efficient Method for the Cytocompatibility Evaluation of Dental Composites

Published on: November 9, 2014

Nanoparticle release from dental composites.

K L Van Landuyt¹, B Hellack, B Van Meerbeek

¹KU Leuven BIOMAT, Department of Oral Health Sciences, University of Leuven & Dentistry, University Hospitals Leuven, Kapucijnenvoer 7, 3000 Leuven, Belgium.

Acta Biomaterialia

|October 15, 2013

Summary

Dental composites release high concentrations of nanoparticles during dental procedures. This airborne nanoparticle dust, measuring 38-70 nm, may pose risks to dental professionals and patients.

Keywords:

Biocompatibility Dental composite Dust Filler Nanoparticle

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Last Updated: May 7, 2026

Confocal Time Lapse Imaging as an Efficient Method for the Cytocompatibility Evaluation of Dental Composites

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Published on: November 9, 2014

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Testing of Nanoparticle Release from a Composite Containing Nanomaterial Using a Chamber System

Testing of Nanoparticle Release from a Composite Containing Nanomaterial Using a Chamber System

Published on: November 22, 2016

Area of Science:

Dental Materials Science
Occupational Health
Nanotoxicology

Background:

Dental composites contain up to 60% nanosized filler particles.
Concerns exist regarding inhalation of nanoparticles by dental personnel and patients during abrasive procedures.

Purpose of the Study:

To investigate the release of airborne nanoparticles from dental composites during clinical procedures.
To characterize the size and composition of released composite dust.

Main Methods:

Exposure measurements of dust in a dental clinic during real work conditions.
Laboratory analysis of composite dust using electron microscopy and energy dispersive X-ray analysis.

Main Results:

High peak concentrations of nanoparticles detected in the breathing zones of dentists and patients.
All tested composites released high concentrations of airborne nanoparticles (>10(6)cm(-3)) with a median diameter of 38-70 nm.
Airborne particles confirmed to be from composite material, consisting of filler particles, resin, or both.

Conclusions:

Dental composite manipulation releases significant concentrations of nanoparticles.
These nanoparticles can be inhaled deeply into the lungs.
Further toxicological research is recommended despite no significant oxidative reactivity observed.