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Simultaneously concentrating and pretreating of microalgae Chlorella spp. by three-phase partitioning
Zhubo Li1, Feifei Jiang, Ya Li
1Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
Bioresource Technology
|October 15, 2013
Summary
Three-phase partitioning (TPP) efficiently concentrates microalgae biomass for biodiesel production. This method enhances lipid extraction yield by 26.3% with lower energy consumption and no impact on lipid quality.
Area of Science:
- Biotechnology
- Renewable Energy
- Algal Research
Background:
- Microalgae are a promising source for sustainable biodiesel.
- Efficient biomass concentration is crucial for cost-effective microalgae processing.
- Traditional methods can be energy-intensive and may affect lipid quality.
Purpose of the Study:
- To evaluate the efficacy of three-phase partitioning (TPP) for concentrating microalgae Chlorella spp.
- To assess the impact of TPP on lipid extraction yield and quality.
- To determine the feasibility of TPP for microalgae biodiesel production.
Main Methods:
- Three-phase partitioning (TPP) was applied to concentrate microalgae Chlorella spp.
- Optimization of TPP parameters including temperature, initial concentration, ethanol to dipotassium hydrogen phosphate (DKP) ratio, and pH.
- Lipid extraction yield and fatty acid profiles were analyzed.
Main Results:
- Over 91.7% of microalgae biomass was concentrated within 10 minutes using TPP.
- Concentration factor was negatively correlated with temperature, initial concentration, and ethanol to DKP ratio.
- Lipid extraction yield increased by 26.3% in TPP-concentrated cells, with no adverse effects on fatty acid profiles.
Conclusions:
- TPP is a rapid, energy-efficient, and effective method for microalgae concentration.
- TPP enhances lipid extraction yield without compromising lipid quality.
- TPP shows significant potential for scalable microalgae biodiesel production.

