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Interaction between the microbial community and invading Escherichia coli O157:H7 in soils from vegetable fields
Zhiyuan Yao1, Haizhen Wang, Laosheng Wu
1Institute of Soil and Water Resources and Environmental Science, Zhejiang Provincial Key Laboratory of Subtropical Soil and Plant Nutrition, Zhejiang University, Hangzhou, China.
Applied and Environmental Microbiology
|October 15, 2013
Summary
Escherichia coli O157:H7 survival in soil negatively impacts microbial communities. Its presence alters bacterial ratios and reduces fungal and actinomycete populations, affecting soil microecology.
Area of Science:
- Environmental microbiology
- Soil science
- Bacteriology
Background:
- Escherichia coli O157:H7 contamination poses risks to food and water safety.
- Limited data exists on the ecological impact of E. coli O157:H7 on soil microbial communities.
Purpose of the Study:
- To investigate the interaction between E. coli O157:H7 and indigenous soil microbial communities.
- To monitor changes in soil microbial structure following E. coli O157:H7 inoculation.
Main Methods:
- Phospholipid fatty acid (PLFA) analysis was employed to assess microbial community composition.
- Simple correlation analysis and principal component analysis were used to evaluate microbial responses.
Main Results:
- E. coli O157:H7 survival was negatively correlated with the Gram-negative to Gram-positive bacterial PLFA ratio (G(-)/G(+)).
- Actinomycete and fungal PLFA levels significantly decreased post-inoculation.
- Invasion by E. coli O157:H7 altered microbial community structure, indicated by changes in G(-)/G(+), bacterial/fungal (B/F), and saturated/monounsaturated (S/M) PLFA ratios.
Conclusions:
- E. coli O157:H7 exerts toxicity not only through pathogenicity but also by disrupting soil microecology.
- The survival and spread of E. coli O157:H7 in soils warrant close attention due to its ecological impact.
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