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Hepatocytes volume regulation.

L Lambotte

    Archives Internationales De Physiologie Et De Biochimie
    |June 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Cell volume regulation in dog livers is maintained by ion exchange, but ouabain disrupts this process. Anoxia also impairs cell volume control, affecting ion balance and hepatocyte volume.

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    Area of Science:

    • Cellular physiology
    • Liver function
    • Ion transport

    Background:

    • Cell volume regulation is crucial for liver function.
    • The sodium-potassium pump (Na/K-ATPase) plays a key role in maintaining cell volume.
    • Ouabain and anoxia are known inhibitors of the sodium pump.

    Purpose of the Study:

    • To investigate the mechanisms of cell volume regulation in isolated dog liver perfusion.
    • To examine the effects of ouabain and anoxia on hepatocyte volume and ion balance.
    • To understand how these factors influence cellular responses to osmotic challenges.

    Main Methods:

    • Isolated dog liver perfusion model.
    • Application of ouabain (10(-4) M) and anoxia to inhibit the sodium pump.
    • Monitoring of intracellular potassium (K) and sodium (Na) concentrations.

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  • Assessment of cellular volume changes under hypotonic and hypertonic conditions.
  • Analysis of chloride (Cl-) gain and membrane potential.

    • Main Results:

    • Ouabain induced a rapid K/Na exchange, maintaining cell volume until intracellular K dropped below 80 mEq/L.
    • Anoxia led to a similar sequence but with a marked excess chloride gain.
    • Hepatocytes subjected to hypotonic shock swelled and partially recovered volume by losing K, maintaining sub-normal volume.
    • Ouabain appeared to inhibit this iso-osmotic regulation.
    • Cells maintained reduced volume in hypertonic medium, even with ouabain present, showing sustained Na/K exchange.

    Conclusions:

    • Cell volume regulation in dog hepatocytes involves complex ion and anion movements.
    • Ouabain and anoxia disrupt normal cell volume homeostasis through distinct but overlapping mechanisms.
    • The study highlights the critical role of the sodium pump and anion balance in protecting hepatocytes against osmotic stress.