Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Common epitopes between mycobacterial and certain host tissue antigens.

C J Thorns, J A Morris

    Clinical and Experimental Immunology
    |August 1, 1985
    PubMed
    Summary

    Monoclonal antibodies (MoAb) targeting Mycobacterium bovis antigens were tested against host tissues. Some MoAb cross-reacted, indicating shared epitopes between Mycobacterium species and host tissues.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Insertion of cobalt into tetrapyrroles.

    Vitamins and hormones·2022
    Same author

    Safety concerns for facial topography customized 3D-printed N95 filtering face-piece respirator produced for the COVID-19 pandemic: initial step is respiratory fit testing.

    International journal of oral and maxillofacial surgery·2020
    Same author

    Pathogenesis of Alzheimer's disease: Multiple interacting causes against which amyloid precursor protein protects.

    Medical hypotheses·2020
    Same author

    Exposure of animals and their products to radiation : surveillance, monitoring and control of national and international trade.

    Revue scientifique et technique (International Office of Epizootics)·2020
    Same author

    United Kingdom.

    Revue scientifique et technique (International Office of Epizootics)·2020
    Same author

    The Lateral Ventricles: A Detailed Review of Anatomy, Development, and Anatomic Variations.

    AJNR. American journal of neuroradiology·2020

    Area of Science:

    • Immunology
    • Microbiology
    • Pathology

    Background:

    • Mycobacterium bovis infection poses a significant threat to animal and human health.
    • Understanding host-pathogen interactions is crucial for developing effective diagnostic and therapeutic strategies.
    • Monoclonal antibodies (MoAb) offer high specificity for identifying microbial and host antigens.

    Purpose of the Study:

    • To investigate the binding specificities of MoAb generated against Mycobacterium bovis sonicated antigen extract.
    • To determine if these MoAb cross-react with common host tissue antigens.
    • To identify potential shared epitopes between Mycobacterium species and host tissues.

    Main Methods:

    • Production of monoclonal antibodies against sonicated Mycobacterium bovis antigen extract.
    • Enzyme-linked immunosorbent assay (ELISA) to assess MoAb binding to 10 common tissue antigens.
    • Reciprocal absorption experiments to confirm antibody specificities.
    • Testing MoAb reactivity with normal and infected guinea pig and ferret tissues.

    Main Results:

    • Five MoAb (MB2, MB3, MB7, MB11, MB13) recognized distinct epitopes on the Mycobacterium bovis antigen extract.
    • MoAb MB3 showed strong reactivity with normal host tissues from infected animals, while MB5 and MB17 did not bind to any tested tissue antigens.
    • Reciprocal absorption experiments validated the observed binding specificities.

    Conclusions:

    • Mycobacterium species possess epitopes that are also present in host tissue antigens.
    • This cross-reactivity could have implications for diagnostic test development and understanding immune responses.
    • Further research is needed to elucidate the precise nature and implications of these shared epitopes.

    Related Experiment Videos