Mapping of type 1 poliovirus neutralization epitopes

Several independently variable neutralization epitopes were identified on type 1 poliovirus by testing various field isolates with a panel of homotypic monoclonal antibodies. According to their distribution among viruses tested, neutralization epitopes were classified as strain-specific or variable (V) and cross-reacting or constant (K). Operational, relationship between different neutralization epitopes was analysed by selecting mutants resistant to neutralization with monoclonal antibodies from wild Mahoney and attenuated Sabin type 1 poliovirus. The results of this study and their implication in the characterization of poliovirus antigenic structure by neutralization epitope formulae is discussed.