Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Coordination of Gene Expression Processes in Bacteria01:29

Coordination of Gene Expression Processes in Bacteria

1.1K
The DNA replication, transcription, and translation processes are intricately coupled in bacteria, allowing efficient gene expression and rapid protein synthesis. While this physical and functional coordination is advantageous, it introduces challenges that bacteria overcome through specific regulatory mechanisms.Coupling of Replication, Transcription, and TranslationThe coupling of replication, transcription, and translation is a hallmark of bacterial gene expression. As the replisome unwinds...
1.1K
Gram-negative Bacterial Protein Secretion Systems01:17

Gram-negative Bacterial Protein Secretion Systems

1.7K
Gram-negative bacteria utilize sophisticated protein secretion systems to transport proteins across their double-membrane envelope into the extracellular environment or host cells. Based on their mechanism of action, these systems are classified into one-step and two-step pathways.One-Step Secretion Systems (Types I, III, IV, and VI)One-step secretion systems bypass the periplasm entirely, forming a continuous channel that spans both the inner and outer membranes:Type I Secretion System (T1SS):...
1.7K
Bacterial Translocation and Protein Secretion01:26

Bacterial Translocation and Protein Secretion

1.2K
Bacterial protein secretion involves translocation systems to ensure proteins reach their designated locations, including the plasma membrane, periplasm, outer membrane, or the external environment. These translocation systems are vital for bacterial physiology, supporting processes like membrane assembly, enzymatic activity in the periplasm, and interactions with the external environment. The division of labor between Sec and Tat pathways ensures efficiency in handling proteins with diverse...
1.2K
Upstream Processing01:27

Upstream Processing

103
Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
103
Bacterial Protein Maturation01:26

Bacterial Protein Maturation

748
Bacterial protein maturation is a tightly regulated process that ensures newly synthesized polypeptides achieve correct functional conformations. This maturation involves a series of modifications, folding events, and quality control steps, often assisted by specialized chaperone proteins.N-Terminal ModificationsThe maturation of bacterial polypeptides begins cotranslationally as the polypeptide exits the ribosome. The first amino acid, N-formylmethionine (fMet), is typically modified at the...
748
Cotranslational Protein Translocation01:20

Cotranslational Protein Translocation

8.4K
Translocation of proteins across membranes is an ancient process that occurs even in bacteria and archaebacteria. In fact, the components of the translocation machinery are still conserved between prokaryotes and eukaryotes.
Sec61 channel partners for cotranslational translocation
During cotranslational translocation, the Sec61 channel partners with the signal recognition particle (SRP), the signal recognition particle receptor (SR), and the ribosomes to transport the nascent polypeptide chain...
8.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Biochemical and functional characterization of the p.A165T missense variant of mitochondrial amidoxime-reducing component 1.

The Journal of biological chemistry·2024
Same author

Comparative Analysis of Multiple Immunoassays for Cytokine Profiling in Drug Discovery.

SLAS discovery : advancing life sciences R & D·2020
Same author

Saccharomyces cerevisiae morphological changes and cytokinesis arrest elicited by hypoxia during scale-up for production of therapeutic recombinant proteins.

Microbial cell factories·2018
Same author

Screen for modulators of atonal homolog 1 gene expression using notch pathway-relevant gene transcription based cellular assays.

PloS one·2018
Same author

Strategies for controlling CRISPR/Cas9 off-target effects and biological variations in mammalian genome editing experiments.

Journal of biotechnology·2018
Same author

Author Correction: Prioritizing multiple therapeutic targets in parallel using automated DNA-encoded library screening.

Nature communications·2018

Related Experiment Video

Updated: May 6, 2026

The Multifaceted Benefits of Protein Co-expression in Escherichia coli
12:48

The Multifaceted Benefits of Protein Co-expression in Escherichia coli

Published on: February 5, 2015

10.8K

Co-expression for intracellular processing in microbial protein production.

Quinn Lu1, Juan C Aon

  • 1Department of Biological Reagents & Assay Development, Platform Technology & Science, GlaxoSmithKline, 1250 South Collegeville Road, Collegeville, PA, 19426, USA, quinn.2.lu@gsk.com.

Biotechnology Letters
|October 17, 2013
PubMed
Summary

Co-expression technology enhances active recombinant protein production by enabling proper protein modification and processing within host cells. This strategy optimizes protein biophysical properties for diverse applications.

More Related Videos

Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX
10:09

Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX

Published on: June 27, 2017

13.0K
Transient Expression and Cellular Localization of Recombinant Proteins in Cultured Insect Cells
12:09

Transient Expression and Cellular Localization of Recombinant Proteins in Cultured Insect Cells

Published on: April 20, 2017

10.5K

Related Experiment Videos

Last Updated: May 6, 2026

The Multifaceted Benefits of Protein Co-expression in Escherichia coli
12:48

The Multifaceted Benefits of Protein Co-expression in Escherichia coli

Published on: February 5, 2015

10.8K
Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX
10:09

Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX

Published on: June 27, 2017

13.0K
Transient Expression and Cellular Localization of Recombinant Proteins in Cultured Insect Cells
12:09

Transient Expression and Cellular Localization of Recombinant Proteins in Cultured Insect Cells

Published on: April 20, 2017

10.5K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Protein Engineering

Background:

  • Recombinant protein activity relies on biophysical properties like post-translational modifications, solubility, and stability.
  • Producing active recombinant proteins necessitates strategic expression and purification methods.

Purpose of the Study:

  • To review co-expression technology for enhancing recombinant protein production.
  • To highlight methods for achieving proper protein modification and processing.

Main Methods:

  • Co-expressing enzymes, folding chaperones, co-factors, and processing enzymes in host cells.
  • Implementing co-translational or post-translational processing strategies.

Main Results:

  • Co-expression facilitates essential modifications for protein activity.
  • Optimized protein processing improves solubility and stability.

Conclusions:

  • Co-expression technology is crucial for producing functional recombinant proteins.
  • This approach offers a versatile strategy for protein engineering and production.