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Updated: May 6, 2026

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals
Published on: August 22, 2019
Biodiversity assessment using hierarchical agglomerative clustering and spectral unmixing over hyperspectral images
Ollantay Medina1, Vidya Manian, J Danilo Chinea
1Computing and Information Sciences and Engineering, University of Puerto Rico at Mayaguez, Call box 9000, Mayaguez 00681, Puerto Rico. ollantay.medina@upr.edu.
Abstract:
Hyperspectral images represent an important source of information to assess ecosystem biodiversity. In particular, plant species richness is a primary indicator of biodiversity. This paper uses spectral variance to predict vegetation richness, known as Spectral Variation Hypothesis. Hierarchical agglomerative clustering is our primary tool to retrieve clusters whose Shannon entropy should reflect species richness on a given zone. However, in a high spectral mixing scenario, an additional unmixing step, just before entropy computation, is required; cluster centroids are enough for the unmixing process. Entropies computed using the proposed method correlate well with the ones calculated directly from synthetic and field data.
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