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Transcriptomic analysis of cut tree peony with glucose supply using the RNA-Seq technique
Chao Zhang1, Yanjie Wang, Jianxin Fu
1College of Landscape Architecture, National Flower Engineering Technology Research Center, Beijing Forestry University, Beijing, 100083, People's Republic of China.
Plant Cell Reports
|October 18, 2013
Summary
Glucose treatment down-regulates ethylene biosynthesis and signaling genes in cut tree peony petals. This sugar supply also represses stress-related transcription factors, potentially enhancing flower longevity.
Area of Science:
- Plant biology
- Molecular biology
- Transcriptomics
Background:
- Tree peony (Paeonia suffruticosa Andrews) is a culturally significant flower.
- Exogenous glucose extends the vase life of cut tree peonies.
- The regulatory mechanisms of sugar on ethylene biosynthesis and signaling in tree peonies remain unclear.
Purpose of the Study:
- To investigate the molecular mechanisms underlying glucose's beneficial effects on cut tree peony flower longevity.
- To analyze transcriptome changes in tree peony petals in response to glucose treatment.
Main Methods:
- Construction of a reference transcriptome from cut tree peony petals.
- Illumina HiSeq™ 2000 sequencing for transcriptome analysis.
- RNA-Seq and real-time quantitative PCR for gene expression validation.
Main Results:
- 33,117 unigenes were annotated.
- 173 differentially expressed genes were identified (41 up-regulated, 132 down-regulated).
- Glucose significantly down-regulated genes encoding ACS (ethylene biosynthesis) and ERF (ethylene signaling).
- Glucose repressed stress-related transcription factors including DREB, CBF, NAC, WRKY, and bHLH.
Conclusions:
- Glucose supply suppresses ethylene biosynthesis and signal transduction pathways in cut tree peony petals.
- Glucose may alleviate stress responses, contributing to prolonged vase life.
- Transcriptome data provides valuable insights into sugar's role in cut flower physiology.
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