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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Updated: May 6, 2026

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Published on: January 7, 2019

DNA microarray to analyze adenovirus-host interactions.

Stefania Piersanti¹, Enrico Tagliafico, Isabella Saggio

¹Dipartimento di Biologia e Biotecnologie, Sapienza, Università di Roma, Roma, Italy.

Methods in Molecular Biology (Clifton, N.J.)

|October 18, 2013

Summary

This summary is machine-generated.

Gene chips identify molecular toxicity of viral vectors, revealing cell cycle changes and immune responses. This protocol aids safe clinical applications of gene therapy vectors.

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A Quantitative Dot Blot Assay for AAV Titration and Its Use for Functional Assessment of the Adeno-associated Virus Assembly-activating Proteins

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Area of Science:

Molecular Biology
Toxicology
Gene Therapy

Background:

Assessing viral vector molecular toxicity is crucial for safe human clinical trials.
DNA chips (gene chips) enable high-throughput analysis of transduced cell profiles.
Previous studies utilized gene chips to identify vector-specific toxicities.

Purpose of the Study:

To detail a protocol for using gene chips to analyze the toxicogenomic signature of helper-dependent adenoviral vectors.
To provide guidelines applicable to various experimental contexts involving viral vector toxicity.
To highlight the importance of in silico data analysis for interpreting gene chip results.

Main Methods:

Utilized DNA chips (gene chips) for high-throughput evaluation of transduced cell profiles.
Applied the protocol to study human and canine helper-dependent adenoviral vectors.
Included detailed procedures for in silico elaboration of gene chip raw data.

Main Results:

Identified vector-specific properties, including cell cycle alterations induced by vector genomic DNA.
Detected activation of specific innate immune pathways attributable to viral particles.
Demonstrated the utility of gene chips in dissecting viral vector toxicogenomic signatures.

Conclusions:

Gene chips are effective tools for defining the molecular toxicity of viral vectors.
The described protocol facilitates the study of adenoviral vector toxicity.
This approach supports the safe clinical translation of gene therapy vectors.