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Updated: Feb 16, 2026
System for Efficacy and Cytotoxicity Screening of Inhibitors Targeting Intracellular Mycobacterium tuberculosis
Published on: April 5, 2017
Yeast three-hybrid screening for identifying anti-tuberculosis drug targets
1Institute of Chemical Science and Engineering, Institute of Bioengineering, National Centre of Competence in Research NCCR Chemical Biology, École Polytechnique Fédérale de Lausanne, EPFL SB ISIC LIP, 1015 Lausanne (Switzerland); Current address: Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139 (USA).
Abstract:
Mycobacterium goes yeast: Target deconvolution of anti-tuberculosis drugs can be a very challenging task. Here we report a yeast 3-hybrid system that allows promising small molecules to be screened for protein targets of a pathogen in nontoxic yeast cells. The system employs libraries of randomly fragmented bacterial DNA and offers a technically simple alternative approach for target identification.
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