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[Effect of salt stress on DNA methylation in Isatis indigotica]
Fei Yang1, Hao Nie, Yan-Hao Xu
1College of Medicine, Yangtze University, Jingzhou 434025, China. yfwhu@163.com
Objective:
To investigate the effect of salt stress on DNA methylation in Isatis indigotica.
Methods:
Methylation sensitive amplification polymorphism (MSAP) approach was used in this study.
Results:
By using 15 pairs of selective primers combinations, a total of 632 MSAP bands were obtained in Isatis indigotica genome under salt stress. In all, 44 (6.9%) CCGG sites displayed cytosine methylation changes under salt stress. Among these sites, 31(4.9%) CCGG sites underwent hypermethylation changes and 13 (2.0%) CCGG sites underwent demethylation changes.
Conclusion:
This result provides evidence for DNA methylation remodeling occurring immediately after salt stress affects Isatis indigotica.
