[Optimization the formulation of Eisemia foetida protein burn spray by response surface methodology]

Area of Science:

• Biochemistry
• Dermatology
• Formulation Science

Context:

• Burn injuries represent a significant global health challenge, necessitating advanced therapeutic strategies.
• Eisemia foetida protein (EFP) has demonstrated potential in wound healing applications.
• Optimizing topical formulations is crucial for maximizing therapeutic efficacy and patient outcomes.

Purpose:

• To determine the optimal formulation parameters for Eisemia foetida protein (EFP) burn spray.
• To evaluate the effect of the optimized EFP formulation on NIH3T3 cell proliferation.

Summary:

• A five-factor, three-level response surface methodology was employed to optimize the EFP burn spray formulation.
• The optimal formulation comprised 0.91% EFP, 1.42% glycerol, and 5% mannitol, with a buffer system (0.02 mol/L Na2HPO4 and 0.01 mol/L citric acid) at pH 7.0.
• This optimized formulation significantly enhanced the proliferation of NIH3T3 cells, as measured by the response variable.

Impact:

• The response surface method proved to be a reliable and efficient tool for optimizing EFP burn spray formulation.
• The findings provide a foundation for the development of a novel and effective EFP-based burn treatment.
• This optimized formulation holds promise for accelerating burn wound healing and improving clinical outcomes.