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Expression and Purification of Virus-like Particles for Vaccination
Published on: June 2, 2016
Viruses associated with influenza-like-illnesses in Papua New Guinea, 2010
Jacinta Kono1, Marinjho H Jonduo, Matthew Omena
1Papua New Guinea Institute of Medical Research, Goroka, Papua New Guinea.
Abstract:
Influenza-like-illness can be caused by a wide range of respiratory viruses. The etiology of influenza-like-illness in developing countries such as Papua New Guinea is poorly understood. The etiological agents associated with influenza-like-illness were investigated retrospectively for 300 nasopharyngeal swabs received by the Papua New Guinea National Influenza Centre in 2010. Real-time PCR/RT-PCR methods were used for the detection of 13 respiratory viruses. Patients with influenza-like-illness were identified according to the World Health Organization case definition: sudden onset of fever (>38°C), with cough and/or sore throat, in the absence of other diagnoses. At least one viral respiratory pathogen was detected in 66.3% of the samples tested. Rhinoviruses (17.0%), influenza A (16.7%), and influenza B (12.7%) were the pathogens detected most frequently. Children <5 years of age presented with a significantly higher rate of at least one viral pathogen and a significantly higher rate of co-infections with multiple viruses, when compared to all other patients >5 years of age. Influenza B, adenovirus, and respiratory syncytial virus were all detected at significantly higher rates in children <5 years of age. This study confirmed that multiple respiratory viruses are circulating and contributing to the presentation of influenza-like-illness in Papua New Guinea.
Insights
Respiratory viruses frequently cause influenza-like-illness (ILI) in Papua New Guinea. Rhinoviruses, influenza A, and influenza B were the most common causes, with young children experiencing higher infection rates.
Area of Science:
- Virology
- Epidemiology
- Public Health
Background:
- Influenza-like-illness (ILI) is a common clinical syndrome with diverse viral etiologies.
- The specific causes of ILI in developing nations like Papua New Guinea remain largely uncharacterized.
- Understanding viral epidemiology is crucial for effective public health interventions.
Purpose of the Study:
- To investigate the etiological agents responsible for influenza-like-illness in Papua New Guinea.
- To identify the prevalence of various respiratory viruses in a cohort of patients presenting with ILI.
- To compare viral detection rates between children and adults.
Main Methods:
- Retrospective analysis of 300 nasopharyngeal swabs collected in 2010.
- Utilized real-time PCR/RT-PCR for the detection of 13 distinct respiratory viruses.
- Applied the World Health Organization case definition for ILI.
Main Results:
- A viral respiratory pathogen was identified in 66.3% of samples.
- Rhinoviruses (17.0%), influenza A (16.7%), and influenza B (12.7%) were the most frequently detected viruses.
- Children under 5 years showed significantly higher rates of viral detection and co-infections, with higher incidences of Influenza B, adenovirus, and respiratory syncytial virus.
Conclusions:
- Multiple respiratory viruses are significant contributors to ILI in Papua New Guinea.
- Young children (<5 years) are disproportionately affected by viral respiratory infections causing ILI.
- The findings highlight the need for targeted surveillance and control strategies for respiratory viruses in the region.
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