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StageTip-based HAMMOC, an efficient and inexpensive phosphopeptide enrichment method for plant shotgun
1RIKEN Center for Sustainable Resource Science, Yokohama, Japan.
Abstract:
Phosphopeptide enrichment is the most critical step for successful LC-MS/MS-based shotgun phosphoproteomics. Recent technological improvements have made selective phosphopeptide enrichment from non-fractionated whole cell lysate digests with a single-step procedure possible. Here, a handy protocol is described for phosphopeptide enrichment from plant materials using hydroxy acid-modified metal oxide chromatography (HAMMMOC) with a stop-and-go-extraction tip (StageTip).
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