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Immunoproteomics analysis of food allergens
1National Institute of Health Sciences (NIHS), Setagaya-ku, Tokyo, Japan.
Methods in Molecular Biology (Clifton, N.J.)
|October 19, 2013
Abstract:
Immunoblot-coupled proteomics based on two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), namely, immunoproteomics, has been used for comprehensive identification of food allergens, because it is a simple and inexpensive tool for rapid identification of several IgE-binding proteins. In this section, we describe our protocols for identification of food allergens using immunoproteomics and discuss a few technical points in detail.
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