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Updated: May 6, 2026

Nanomanipulation of Single RNA Molecules by Optical Tweezers

Nanomanipulation of Single RNA Molecules by Optical Tweezers

Published on: August 20, 2014

RNA folding dynamics using laser-assisted single-molecule refolding.

Bishnu Paudel¹, David Rueda

¹Department of Medicine, Section of Virology, Imperial College, London, UK.

Methods in Molecular Biology (Clifton, N.J.)

|October 19, 2013

Summary

This summary is machine-generated.

We present detailed protocols for Laser-assisted single-molecule refolding (LASR), a technique combining temperature-jump kinetics and single-molecule detection to study complex RNA folding pathways and misfolded intermediates.

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Thermodynamics of Membrane Protein Folding Measured by Fluorescence Spectroscopy

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Published on: April 28, 2011

Area of Science:

Biochemistry and Molecular Biology
Biophysics

Background:

RNA folding pathways are complex, often involving kinetic traps and slow-to-resolve misfolded intermediates.
Understanding these pathways is crucial for elucidating RNA molecule biological functions.

Purpose of the Study:

To provide detailed protocols for performing Laser-assisted single-molecule refolding (LASR) experiments.
To enable researchers to characterize complex RNA folding pathways and misfolded intermediates.

Main Methods:

Laser-assisted single-molecule refolding (LASR) combines temperature-jump (T-jump) kinetics with single-molecule detection.
Single-molecule fluorescence resonance energy transfer (smFRET) is used to characterize conformational dynamics in real-time.
Protocols cover sample preparation, temperature calibration, and data analysis for LASR experiments.

Main Results:

Detailed protocols for LASR experiments are provided.
The protocols facilitate the characterization of RNA conformational dynamics.
The methods allow for the study of kinetic traps and misfolded intermediates in RNA folding.

Conclusions:

The provided LASR protocols offer a robust method for investigating complex RNA folding dynamics.
This technique is valuable for understanding how RNA molecules achieve their functional states.
Researchers can now more effectively characterize misfolded RNA intermediates and folding pathways.