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Published on: February 12, 2022
Exploring RNA oligomerization and ligand binding by fluorescence correlation spectroscopy and small angle X-ray
Eileen Magbanua1, Petr V Konarev, Dmitri I Svergun
1MIN Faculty, Chemistry Department, Institute for Biochemistry and Molecular Biology, Hamburg University, Hamburg, Germany.
Abstract:
RNA forms defined structures and binds specifically to target molecules. The combination of data which results from fluorescence correlation spectroscopy (FCS) and small angle X-ray scattering (SAXS) measurements can be used to determine intermolecular interactions between RNA and its binding partners. To define oligomerization states of free RNA and its complexes with bound target molecules, hydrodynamic radii, radii of gyration as well as the maximum sizes of the components have to be determined and compared. Furthermore, the program OLIGOMER allows calculating the portions of monomeric and dimeric RNA, for instance, within a mixture.
