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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: May 6, 2026

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM

Published on: June 28, 2017

MRT letter: light sheet based imaging flow cytometry on a microfluidic platform.

Raju Regmi¹, Kavya Mohan, Partha P Mondal

¹Nanobioimaging Laboratory, Department of Instrumentation and Applied Physics, Indian Institute of Science, Bangalore, 560012, India.

Microscopy Research and Technique

|October 19, 2013

Summary

This summary is machine-generated.

We developed a novel light sheet imaging flow cytometry method for cell counting and imaging on microfluidic devices. This technique offers high throughput and reduced background noise, advancing microfluidic cytometry applications.

Keywords:

cytometry fluorescence imaging light-sheet microscopy

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Area of Science:

Biomedical Engineering
Microfluidics
Optical Imaging

Background:

Traditional flow cytometry requires complex flow focusing and scanning, limiting throughput and increasing background noise.
Existing microfluidic cytometry platforms often struggle with efficient cell detection and imaging.
Developing cost-effective and high-throughput cell analysis tools is crucial for diagnostics.

Discussion:

The proposed light sheet imaging flow cytometry eliminates the need for flow focusing and point scanning by illuminating the entire microfluidic channel.
An orthogonal detection geometry effectively blocks incident light, significantly minimizing background noise.
This approach enables simultaneous cell counting and on-the-go imaging within the microfluidic channel.

Key Insights:

Achieved high cell counting throughput of 2,090 cells/min using fluorescently coated Saccharomyces cerevisiae cells.
Demonstrated simultaneous imaging of individual cells at low flow rates.
The system is cost-effective with a simple microfluidic channel design.

Outlook:

This technique holds promise for advancing microfluidic-based cytometry.
Potential applications include nanomedicine and point-of-care diagnostics.
Further development could lead to more accessible and efficient cell analysis tools.