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Genotoxic effect of two bleaching agents on oral mucosa
Eva Klaric1, Matej Par, Ivona Profeta
1Department of Endodontics and Restorative Dentistry, School of Dental Medicine, University of Zagreb, Gunduliceva 5, 10000 Zagreb, Croatia. eklaric@sfzg.hr.
Aim:
To analyze the genotoxic effect of two hydrogen peroxide-containing bleaching products on oral mucosal cells.
Materials And Methods:
The research was conducted on 22 individuals divided into two groups. Group 1 used ZOOM2 and group 2 the Opalescence BOOST bleaching agent. Specimens of the gingival and the upper lip mucosa were obtained before, immediately after, and 72 h after the bleaching procedure and were analyzed using a micronucleus test.
Results:
Seventy-two hours after bleaching treatment with BOOST, samples collected from the oral mucosa exhibited a statistically significant increase of all genotoxicity markers, with large effect sizes (Cohen's d>0.8) observed in the total number of micronuclei (MN), number of cells with 3+ MN, karyolysis and bi-nuclear cells. ZOOM2 treatment showed a significant increase, with medium-to-large effect sizes, in the number of cells with 1 MN, karyolysis, nuclear buds and bi-nuclear cells.
Conclusion:
Both preparations demonstrated potential genotoxic effects.
