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Updated: May 6, 2026
Sample Preparation for Probe Electrospray Ionization Mass Spectrometry
Published on: February 19, 2020
A rapid and sensitive LC-ESI-MS/MS method for the detection of YF-49-92.MLS in rat plasma
Qin Gao1, Xin Huang, Zhenzhou Jiang
1Jiangsu Center for Drug Screening, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, China.
Background:
YF-49-92.MLS is a novel candidate for TB treatment. An accurate, precise and specific LC-MS/MS method for the quantification of YF-49-92.MLS in rat plasma using verapamil as an IS is reported in this paper.
Methodology:
Proper retention time and excellent peak shape were acquired using an Agilent Zorbax(®) SB-C18 column with the mobile phase of 5 mmol/l ammonium acetate, 0.1% formic acid-methanol (30:70, v/v). The LLOQ was 1 ng/ml. The calibration curves encompassed concentrations from 20 to 5000 ng/ml. Intra- and inter-assay precision and accuracy were within 15% by determining low, medium and high concentration samples. Extraction recovery, stability, and matrix effects were also fully validated.
Conclusion:
This method has been validated to be rapid and sensitive, and successfully applied to the PK study of YF-49-92.MLS in rat plasma.
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