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Protons decrease the single channel conductance of the sarcoplasmic reticulum K+ channel in neutral and negatively

J Bell

    Biophysical Journal
    |August 1, 1985
    PubMed
    Summary
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    Protons inhibit potassium (K+) channel conductance in rabbit sarcoplasmic reticulum by competitively binding. This effect is accurately modeled by modified Gouy-Chapman-Stern theory in charged membranes.

    Area of Science:

    • Biophysics
    • Membrane Physiology
    • Ion Channel Function

    Background:

    • Sarcoplasmic reticulum potassium channels are crucial for muscle function.
    • Understanding ion channel conductance is vital for cellular electrophysiology.
    • Proton (H+) interactions can modulate channel activity.

    Purpose of the Study:

    • To investigate the effect of protons on rabbit sarcoplasmic reticulum K+ channel conductance.
    • To determine the inhibitory constant (Ki) of protons on K+ channels.
    • To model K+ channel conductance in artificial bilayers using modified theories.

    Main Methods:

    • Incorporation of rabbit sarcoplasmic reticulum K+ channels into artificial lipid bilayers.
    • Measurement of channel conductance under varying K+ and proton concentrations.

    Related Experiment Videos

  • Application of modified Gouy-Chapman-Stern theory for data analysis.

    • Main Results:

    • Protons competitively inhibit K+ channel conductance with a Ki of 0.5 microM.
    • Conductance in negatively charged membranes is well-described by modified Gouy-Chapman-Stern theory.
    • A channel mouth isolation of 5-10 A from the bilayer surface was inferred.

    Conclusions:

    • Proton inhibition is a significant factor affecting K+ channel function.
    • The Gouy-Chapman-Stern model, with modifications, accurately predicts channel behavior in charged membranes.
    • Channel mouth geometry influences ion conductance and proton interaction.