Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Thermally Activated Vaporization of Fluorocarbon-In-Hydrocarbon Exoskeletal Droplets.

ACS omega·2026
Same author

A model for a lipid-coated microbubble based on transient network theory.

The Journal of the Acoustical Society of America·2026
Same author

Radiation induced vaporization of exoskeletal droplets as potential x-ray acoustic contrast agents.

Medical physics·2025
Same author

Frequency-Selective Microbubble Targeting <i>In Vitro</i>: A Step Toward Multicolor Ultrasound Molecular Imaging.

ACS applied bio materials·2025
Same author

Peritoneal Infusion of Oxygen Microbubbles Alters the Metabolomic Profile of the Lung and Spleen in Acute Hypoxic Exposure.

Bioengineering (Basel, Switzerland)·2024
Same author

Effect of Poly(ethylene glycol) Configuration on Microbubble Pharmacokinetics.

ACS biomaterials science & engineering·2024

Related Experiment Video

Updated: May 6, 2026

Multi-timescale Microscopy Methods for the Characterization of Fluorescently-labeled Microbubbles for Ultrasound-Triggered Drug Release
06:02

Multi-timescale Microscopy Methods for the Characterization of Fluorescently-labeled Microbubbles for Ultrasound-Triggered Drug Release

Published on: June 12, 2021

4.8K

Single-particle optical sizing of microbubbles.

Scott J Satinover1, Jacob D Dove, Mark A Borden

  • 1Department of Mechanical Engineering, University of Colorado, Boulder, Colorado, USA.

Ultrasound in Medicine & Biology
|October 22, 2013
PubMed
Summary

Generalized Lorenz-Mie scattering theory (GLMT) combined with optical scatter measurements accurately determines microbubble size. This method overcomes limitations of light obscuration techniques for microbubble ultrasound contrast agents.

Keywords:
Electro-impedance sensingFlow cytometryGeneralized Lorenz-Mie theoryLaser obscuration/extinctionLipid-coated microbubblesPolystyrene beads

More Related Videos

Imaging and Quantification of the Area of Fast-Moving Microbubbles Using a High-Speed Camera and Image Analysis
05:31

Imaging and Quantification of the Area of Fast-Moving Microbubbles Using a High-Speed Camera and Image Analysis

Published on: September 5, 2020

6.8K
Fabricating and Labeling Microbubbles with Fluorescent and Radioactive Tracers
10:40

Fabricating and Labeling Microbubbles with Fluorescent and Radioactive Tracers

Published on: January 24, 2025

1.2K

Related Experiment Videos

Last Updated: May 6, 2026

Multi-timescale Microscopy Methods for the Characterization of Fluorescently-labeled Microbubbles for Ultrasound-Triggered Drug Release
06:02

Multi-timescale Microscopy Methods for the Characterization of Fluorescently-labeled Microbubbles for Ultrasound-Triggered Drug Release

Published on: June 12, 2021

4.8K
Imaging and Quantification of the Area of Fast-Moving Microbubbles Using a High-Speed Camera and Image Analysis
05:31

Imaging and Quantification of the Area of Fast-Moving Microbubbles Using a High-Speed Camera and Image Analysis

Published on: September 5, 2020

6.8K
Fabricating and Labeling Microbubbles with Fluorescent and Radioactive Tracers
10:40

Fabricating and Labeling Microbubbles with Fluorescent and Radioactive Tracers

Published on: January 24, 2025

1.2K

Area of Science:

  • Acoustics
  • Optical Physics
  • Biomedical Engineering

Background:

  • Single-particle optical sizing is crucial for characterizing microbubble ultrasound contrast agents.
  • Understanding microbubble dynamics under ultrasound stimulation requires accurate size distribution data.
  • Current optical sizing methods face challenges in accurately representing microbubble populations.

Purpose of the Study:

  • To compare experimental light obscuration and scattering measurements with generalized Lorenz-Mie scattering theory (GLMT) predictions for microbubble size distributions.
  • To investigate the accuracy of GLMT in predicting microbubble behavior during ultrasound stimulation.
  • To establish a reliable method for determining microbubble size distributions using optical techniques.

Main Methods:

  • Utilized single-particle optical sizing techniques, including light obscuration and scattering.
  • Applied generalized Lorenz-Mie scattering theory (GLMT) for theoretical calculations.
  • Employed polymer bead standards to establish conversion factors between GLMT and experimental flow cytometry data.
  • Measured microbubble size distributions and scattering plots using flow cytometry.

Main Results:

  • Demonstrated that single-particle light obscuration can misrepresent mono-modal size distributions as multi-modal due to non-linearities.
  • Established conversion factors using polymer bead standards for accurate GLMT application.
  • Showed that GLMT calculations, with conversion factors, accurately predict experimental flow cytometry scattering plots for microbubbles.
  • Validated the use of optical forward and side scatter measurements with GLMT.

Conclusions:

  • Generalized Lorenz-Mie scattering theory (GLMT) is a robust framework for microbubble optical sizing.
  • Combining GLMT with optical scatter measurements provides accurate microbubble size determination.
  • This approach overcomes limitations of traditional light obscuration methods for microbubble characterization.
  • The findings support the use of GLMT-enhanced optical methods for microbubble ultrasound contrast agents.