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Profiling polyphenols in five Brassica species microgreens by UHPLC-PDA-ESI/HRMS(n.)
Jianghao Sun1, Zhenlei Xiao, Long-Ze Lin
1Food Composition and Methods Development Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, U.S. Department of Agriculture , 10300 Baltimore Avenue, Beltsville, Maryland 20705, United States.
Abstract:
Brassica vegetables are known to contain relatively high concentrations of bioactive compounds associated with human health. A comprehensive profiling of polyphenols from five Brassica species microgreens was conducted using ultrahigh-performance liquid chromatography photodiode array high-resolution multistage mass spectrometry (UHPLC-PDA-ESI/HRMS(n)). A total of 164 polyphenols including 30 anthocyanins, 105 flavonol glycosides, and 29 hydroxycinnamic acid and hydroxybenzoic acid derivatives were putatively identified.The putative identifications were based on UHPLC-HRMS(n) analysis using retention times, elution orders, UV-vis and high-resolution mass spectra, and an in-house polyphenol database as well as literature comparisons. This study showed that these five Brassica species microgreens could be considered as good sources of food polyphenols.
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