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Decrease of potassium permeability by intracellular application of sodium ions in snail neurons.

J Walden, O W Witte, E J Speckmann

    Neuroscience Letters
    |September 30, 1985
    PubMed
    Summary
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    Intracellular sodium (Na+) injection in Helix pomatia neurons primarily caused an inward current, suggesting potassium channel blockade. A small outward current indicated a calcium-activated potassium current.

    Area of Science:

    • Neuroscience
    • Cellular Electrophysiology
    • Ion Channel Function

    Background:

    • Neurons B1, B2, and B3 in Helix pomatia are key models for studying neuronal excitability.
    • Understanding ion channel behavior is crucial for comprehending neuronal signaling.

    Purpose of the Study:

    • To investigate the effects of intracellular ion injections on neuronal membrane currents in Helix pomatia.
    • To characterize the nature of induced inward and outward currents and their underlying mechanisms.

    Main Methods:

    • Intracellular injection of sodium (Na+), lithium (Li+), and Tris+ into Helix pomatia neurons.
    • Measurement of membrane potential and conductance changes.
    • Application of tetraethylammonium (TEA) and quinine to assess ion channel involvement.

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    Main Results:

    • Intracellular Na+ induced outward currents in 10% and inward currents in 90% of experiments.
    • Outward currents increased membrane conductance; inward currents decreased it.
    • Both currents reversed between -60 and -70 mV. Inward currents were inhibited by TEA and quinine.

    Conclusions:

    • The outward current is likely a calcium-activated potassium current.
    • The predominant inward current suggests intracellular blockade of potassium channels by Na+ or other injected cations.