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Updated: May 6, 2026

A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules
Published on: March 10, 2017
[Phenotypic screens or one stone to kill two birds: discover the target and its pharmacological regulator]
Renaud Prudent1, Emmanuelle Soleilhac, Caroline Barette
1Institut Albert Bonniot, CRI Inserm/Université Joseph Fourier (UJF) U823, équipe 3 Polarité, développement et cancer, rond-point de la Chantourne, 38706 La Tronche Cedex, France.
Abstract:
Phenotypic screens, in which chemical libraries are assayed on cells with the aim to isolate compounds that interfere with a given cell function, are a risky but powerful strategy to discover, in the same approach, new therapeutic targets and the compounds able to regulate them. With a strong experience of nearly 10 years in the field, we present the advantages of such an approach, the possible troubles and technical solutions. We also present in this paper a french network which has been recently built and that gather all the competencies needed for screening approaches.
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