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Related Experiment Video

Updated: May 6, 2026

Perfusable Vascular Network with a Tissue Model in a Microfluidic Device

Perfusable Vascular Network with a Tissue Model in a Microfluidic Device

Published on: April 4, 2018

Control of perfusable microvascular network morphology using a multiculture microfluidic system.

Jordan A Whisler¹, Michelle B Chen, Roger D Kamm

¹1 Department of Mechanical Engineering, Massachusetts Institute of Technology , Cambridge, Massachusetts.

Tissue Engineering. Part C, Methods

|October 25, 2013

Summary

This summary is machine-generated.

Researchers engineered vascular networks using microfluidics, controlling cell interactions and material properties. This allows for precise design of microvascular networks (MVNs) for tissue engineering applications.

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Last Updated: May 6, 2026

Perfusable Vascular Network with a Tissue Model in a Microfluidic Device

Perfusable Vascular Network with a Tissue Model in a Microfluidic Device

Published on: April 4, 2018

Micropatterning and Assembly of 3D Microvessels

Micropatterning and Assembly of 3D Microvessels

Published on: September 9, 2016

Endothelialized Microfluidics for Studying Microvascular Interactions in Hematologic Diseases

Endothelialized Microfluidics for Studying Microvascular Interactions in Hematologic Diseases

Published on: June 22, 2012

Area of Science:

Biomaterials Engineering
Cell Biology
Microfluidics

Background:

The microenvironment critically impacts microvascular network (MVN) formation by endothelial cells (ECs).
Understanding these influences is key for engineering vascular networks with specific morphologies.

Purpose of the Study:

To quantify how microenvironmental factors dictate MVN parameters.
To establish design principles for engineering vascular networks with controlled morphologies.

Main Methods:

Developed a multiculture microfluidic platform for precise control over paracrine signaling, cell densities, and hydrogel mechanics.
Co-cultured human umbilical vein endothelial cells (HUVECs) with human lung fibroblasts (HLFs) in fibrin gels.

Main Results:

Engineered vessels exhibited patent, perfusable lumens.
Cell-to-cell communication was essential for network stability beyond 4 days.
Network parameters like branching, vascularized area, and vessel diameter were uniquely dependent on multiple input factors.

Conclusions:

Specific inputs, such as angiogenic growth factors (VEGF, S1P) or fibrinogen concentration, can modulate vessel diameter.
Findings provide control over MVN design for tissue-specific engineering applications.