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Related Experiment Video

Updated: May 1, 2026

Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling |

Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling |

Published on: November 13, 2011

Dissecting organ-specific transcriptomes through RNA-sequencing.

Shan Guan, Yingqing Lu¹

¹State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, 20 Nan Xin Cun, Xiangshan, Beijing 100093, China. yqlu@ibcas.ac.cn.

|October 26, 2013

Summary

This summary is machine-generated.

Next-generation sequencing (NGS) reveals organ-specific gene expression in Ipomoea. This transcriptomic data provides insights into developmental processes and metabolic pathways, suggesting petals evolved from leaves.

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Area of Science:

Plant biology
Genomics
Molecular networks

Background:

Organ-specific gene expression offers insights into biological processes.
Microarray profiling previously aided understanding of mutants, signaling, and development.
Next-generation sequencing (NGS) provides more detailed gene expression data.

Purpose of the Study:

To analyze transcriptomic variations within and between Ipomoea organs.
To infer developmental processes using NGS data and bioinformatic tools.
To explore connections between morphological traits and molecular networks.

Main Methods:

Collected transcriptomic data using a Solexa/Illumina platform.
Analyzed transcriptomic variations within and between leaf, sepal, and petal samples.
Reconstructed metabolic networks for inferring substance flow.

Main Results:

Retrieved in vivo transcriptome signals using NGS, data handling, and bioinformatics.
Observed a decrease in transcribed genes from leaf to sepal to petal.
Found petal transcriptomes resemble leaf, with chloroplast transcripts as a subset.
Inferred sucrose to anthocyanin synthesis pathway in petal development.

Conclusions:

Developmental transcriptomes are informative for linking morphology and molecular networks.
Hypothesized petal as a derived leaf organ.
Suggested petal color modification via metabolic network substance flow.