Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

8.5K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
8.5K
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

2.4K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
2.4K
Nucleotide Excision Repair01:38

Nucleotide Excision Repair

4.7K
DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
4.7K
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

33.7K
Overview
33.7K
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

11.0K
11.0K
RNA Interference01:23

RNA Interference

24.4K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
24.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Therapeutic inhibition of telomeric DNA damage response rescues hematopoietic dysfunction driven by telomere shortening and aging.

Nature aging·2026
Same author

Site-specific DNA double-strand break induces local transcription in cis and protein expression.

Communications biology·2026
Same author

Toward actionable interventions in human aging (12th ARDD meeting, 2025).

Aging·2026
Same author

Combined physical and cognitive training enhances telomere length in mild cognitive impairment patients.

BMC geriatrics·2026
Same author

Degrees of H2AX phosphorylation correlate with unique features of the intratumoral immune microenvironment in colorectal carcinomas.

The oncologist·2026
Same author

Molecular evolution of animal aging.

The EMBO journal·2026

Related Experiment Video

Updated: May 6, 2026

Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage
10:44

Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage

Published on: January 31, 2018

9.8K

A direct role for small non-coding RNAs in DNA damage response.

Fabrizio d'Adda di Fagagna1

  • 1Istituto Firc di Oncologia Molecolare (IFOM) Foundation - Fondazione Italiana per la Ricerca sul Cancro (FIRC) Institute of Molecular Oncology Foundation, Via Adamello 16, 20139 Milan, Italy; Istituto di Genetica Molecolare - Consiglio Nazionale delle Ricerche, Via Abbiategrasso 207, 27100 Pavia, Italy.

Trends in Cell Biology
|October 26, 2013
PubMed
Summary

Small non-coding RNAs (ncRNAs) are newly discovered regulators of DNA integrity. These DNA damage-response RNAs (DDRNAs) are generated at DNA damage sites to control the DNA damage response and are relevant in cancer.

Keywords:
DDRNADICERDNA damage response (DDR)DROSHAdiRNAnon-coding RNA (ncRNA)

More Related Videos

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
10:59

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage

Published on: August 21, 2021

2.8K
Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

10.1K

Related Experiment Videos

Last Updated: May 6, 2026

Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage
10:44

Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage

Published on: January 31, 2018

9.8K
Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
10:59

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage

Published on: August 21, 2021

2.8K
Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

10.1K

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Cellular RNA historically played a secondary role to DNA, primarily in protein synthesis (mRNA, tRNA, rRNA).
  • Non-protein-coding RNAs (ncRNAs) emerged as regulators of gene expression.
  • Emerging evidence highlights small ncRNAs in maintaining DNA integrity.

Purpose of the Study:

  • To review the emerging role of small ncRNAs in the DNA damage response (DDR).
  • To discuss the biogenesis, mechanisms, and cancer relevance of these novel RNA molecules.

Main Methods:

  • Literature review of studies investigating small ncRNAs at DNA damage sites.
  • Analysis of evidence for the function of DNA damage-response RNAs (DDRNAs) or double-strand break (DSB)-induced RNAs (diRNAs).

Main Results:

  • Small ncRNAs, termed DDRNAs or diRNAs, are generated at DNA damage sites.
  • These ncRNAs signal DNA damage and control the DNA damage response (DDR).
  • Their biogenesis and mechanisms of action are crucial for accurate DNA repair.

Conclusions:

  • Small ncRNAs play a critical role in safeguarding DNA integrity.
  • DDRNAs are key players in the cellular response to DNA damage.
  • Dysregulation of DDRNAs may contribute to cancer development.