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Immunogenicity of a bovine rotavirus glycoprotein fragment
Abstract:
Previous experiments demonstrated that an antigenic site responsible for virus neutralization and cell attachment was located on a 14,000-molecular-weight fragment of the major bovine rotavirus (BRV) glycoprotein (M. Sabara, J. E. Gilchrist, G. R. Hudson, and L. A. Babiuk, J. Virol. 53:58-66, 1985). However, it was necessary to investigate whether this fragment also had the ability to induce the production of neutralizing antibodies. Upon immunization of mice, the bovine serum albumin-conjugated 14,000-molecular-weight fragment, the unconjugated 14,000-molecular-weight fragment, and the native glycoprotein all induced a similar neutralizing antibody response, albeit to a lesser extent than did the infectious, whole virus. In addition, immuno-blot enzyme-linked immunosorbent assay analysis of the reactivity of anti-peptide serum versus anti-glycoprotein serum with the glycoprotein was very comparable. These results suggest that the 14,000-molecular-weight fragment may represent not only a biologically active region but also an immunodominant area of the glycoprotein.
Insights
A bovine rotavirus (BRV) glycoprotein fragment induces neutralizing antibodies, suggesting it
Area of Science:
- Veterinary Virology
- Immunology
- Molecular Biology
Background:
- Previous studies identified a 14,000-molecular-weight fragment of the major bovine rotavirus (BRV) glycoprotein as an antigenic site.
- This fragment was previously shown to be responsible for virus neutralization and cell attachment.
Purpose of the Study:
- To investigate if the 14,000-molecular-weight glycoprotein fragment can induce the production of neutralizing antibodies.
- To determine if this fragment is immunodominant.
Main Methods:
- Immunization of mice with the 14,000-molecular-weight fragment (conjugated and unconjugated) and the native glycoprotein.
- Analysis of neutralizing antibody response.
- Immuno-blot enzyme-linked immunosorbent assay (ELISA) to compare antibody reactivity.
Main Results:
- All tested forms of the glycoprotein fragment induced a neutralizing antibody response in mice.
- The antibody response was comparable between conjugated and unconjugated fragments.
- The response was less potent than that induced by the whole infectious virus.
- Immuno-blot ELISA showed comparable reactivity between anti-peptide and anti-glycoprotein sera.
Conclusions:
- The 14,000-molecular-weight fragment of the BRV glycoprotein is capable of inducing a neutralizing antibody response.
- This fragment represents a biologically active and immunodominant region of the glycoprotein.
- The findings support the potential use of this fragment in vaccine development or diagnostic tools.