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DNA Methylation: Bisulphite Modification and Analysis
Published on: October 21, 2011
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Methyl-combing: single-molecule analysis of DNA methylation on stretched DNA fibers
1Biochemistry Center Regensburg, University of Regensburg, Regensburg, Germany.
Methods in Molecular Biology (Clifton, N.J.)
|October 29, 2013
Abstract:
The methyl-combing technique combines the dynamic molecular combing method with the detection of DNA modifications. The assay allows the single-molecule analysis of epigenetic marks on regularly stretched DNA fibers, at the megabase scale with kilobase resolution. The protocol presented in this chapter is based on proof-of-principle experiments where the single-molecule detection of DNA methylation has been performed on unmethylated and in vitro methylated λ phage DNA.
