Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Lens junctions are communicating junctions.

C Peracchia, S J Girsch, G Bernardini

    Current Eye Research
    |November 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    How distant? An experimental analysis of students' COVID-19 exposure and physical distancing in university buildings.

    International journal of disaster risk reduction : IJDRR·2022
    Same author

    Multiple gene expression profiling suggests epithelial dysfunction in polypoid chronic rhinosinusitis.

    Acta otorhinolaryngologica Italica : organo ufficiale della Societa italiana di otorinolaringologia e chirurgia cervico-facciale·2019
    Same author

    Inflammatory and oxidative stress biomarkers in alkaptonuria: data from the DevelopAKUre project.

    Osteoarthritis and cartilage·2018
    Same author

    Surface properties of nanocrystalline TiO2 coatings in relation to the in vitro plasma protein adsorption.

    Biomedical materials (Bristol, England)·2015
    Same author

    Distinctive gene expression profiles in Balb/3T3 cells exposed to low dose cobalt nanoparticles, microparticles and ions: potential nanotoxicological relevance.

    Journal of biological regulators and homeostatic agents·2013
    Same author

    Fish welfare and genomics.

    Fish physiology and biochemistry·2011
    Same journal

    Current Experimental Methods for Restoration of the Tear Film.

    Current eye research·2026
    Same journal

    Apolipoprotein C3 Promotes Retinal Angiogenesis via the MYC Pathway in Hypoxia.

    Current eye research·2026
    Same journal

    Possible Causal Association Between Thyroid-Related Traits and Diabetic Retinopathy Risk: Evidence From 23 Medication-Taking Traits.

    Current eye research·2026
    Same journal

    Linking Microstructure to Mechanics in ICG-Stained Lens Capsules: Insights from Nanoindentation to Electron Microscopy.

    Current eye research·2026
    Same journal

    Corneal Sensitivity, Sub-Basal Corneal Nerve Plexus, and Tear Neuropeptides in Symptomatic Versus Asymptomatic Visual Display Terminal Users.

    Current eye research·2026
    Same journal

    Selective Laser Trabeculoplasty Mitigates Dexamethasone-Induced Myofibroblast-Like Transformation and Dysfunction in Human Trabecular Meshwork Cells.

    Current eye research·2026
    See all related articles

    Lens fibers communicate via unique junctions, not typical gap junctions. The lens junction protein MIP26 forms channels, suggesting a specialized cell communication system in the eye lens.

    Area of Science:

    • Ophthalmology
    • Cell Biology
    • Biophysics

    Background:

    • Lens fibers are electrically coupled, exchanging molecules.
    • This communication typically occurs via gap junctions in most cells.
    • Lens fibers, however, lack typical gap junctions, possessing unique lens junctions.

    Purpose of the Study:

    • To investigate the nature of cell-to-cell communication in lens fibers.
    • To characterize the lens junction protein MIP26 and its channel-forming properties.
    • To understand the unique transport requirements of the eye lens.

    Main Methods:

    • Morphological, chemical, and immunological comparisons of lens junctions to gap junctions.
    • Incorporation of MIP26 into liposomes to assess channel formation and permeability.

    Related Experiment Videos

  • Investigating the effects of Ca++, H+, and calmodulin (CaM) on channel gating.
  • Analyzing the impact of MIP26 C-terminal arm cleavage on channel function.

    • Main Results:

    • Lens junctions, while structurally similar to gap junctions, are distinct.
    • MIP26 forms channels permeable to molecules up to 1.5 kDa.
    • Lens junctions exhibit Ca++-dependent uncoupling and channel closure with Ca++/H+/CaM.
    • Proteolytic cleavage of MIP26's C-terminal arm impairs gating competency.

    Conclusions:

    • Lens junctions function as communicating junctions, despite lacking typical gap junction structures.
    • MIP26 is a key protein forming these unique channels.
    • The lens may possess specialized transport needs necessitating these distinct junctions.