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Developmental changes in keratin patterns during epidermal maturation.

T R Ellison, P M Mathisen, L Miller

    Developmental Biology
    |December 1, 1985
    PubMed
    Summary
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    This study identifies distinct keratin protein groups during Xenopus laevis tadpole development. Embryonic keratins disappear during metamorphosis, while adult epidermal keratins emerge and accumulate.

    Area of Science:

    • Developmental Biology
    • Cell Biology
    • Biochemistry

    Background:

    • The epidermis undergoes significant biochemical changes during development.
    • Keratins are key structural proteins in epithelial tissues, with expression patterns varying across species and developmental stages.
    • Understanding keratin expression in Xenopus laevis provides insights into vertebrate epidermal development.

    Purpose of the Study:

    • To identify and characterize keratin expression during the biochemical maturation of the Xenopus laevis epidermis.
    • To compare developmental keratin profiles with those of adult epidermis and non-epidermal cell lines.
    • To classify keratins based on their expression patterns throughout development and metamorphosis.

    Main Methods:

    • Analysis of keratin protein expression at various developmental stages of Xenopus laevis.

    Related Experiment Videos

  • Comparison of keratin profiles from embryonic epidermis, adult epidermis, and two non-epidermal epithelial cell lines.
  • Biochemical identification and molecular weight determination of expressed keratins.

    • Main Results:

    • Three classes of keratins were identified: embryo-specific (58, 59 kDa), developmentally prominent but minor in adults (47, 48, 60 kDa), and adult epidermal major components (49, 53, 56, 63 kDa).
    • Embryo-specific keratins are present before metamorphosis and disappear during epidermal keratinization.
    • Class 1 and 2 keratins are found in non-epidermal cell lines, while Class 3 keratins show limited overlap with these cell lines.

    Conclusions:

    • Xenopus laevis epidermal development involves a dynamic regulation of keratin expression.
    • Embryonic keratins are replaced by adult-specific keratins during metamorphosis, reflecting functional and structural maturation.
    • Distinct keratin repertoires characterize different epithelial cell types and developmental stages in Xenopus laevis.