Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Advances in Brain Tumor Biomarkers: From Molecular Profiling to Liquid Biopsy and AI-Driven Detection.

Cancers·2026

Same author

Exploiting Synthetic Lethality of PRMT5 for Precision Treatment of MTAP-Deficient Glioblastoma.

International journal of translational medicine (Basel, Switzerland)·2026

Same author

SCD1 and SCD5 modulate PARP-dependent DNA repair via fatty acid desaturation in glioblastoma.

Cell reports·2026

Same author

Dual-action nanotherapy: Temozolomide-loaded, anti-PD-L1 scFv-functionalized lipid nanocarriers for targeted glioblastoma therapy.

European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences·2025

Same author

Current and Emerging Fluorescence-Guided Techniques in Glioma to Enhance Resection.

Cancers·2025

Same author

SCD1 and SCD5 Modulate PARP-Dependent DNA Repair via Fatty Acid Desaturation in Glioblastoma.

bioRxiv : the preprint server for biology·2025

Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Methods for Functional Validation of Terpenoid Metabolic Clusters in Nicotiana benthamiana and Aspergillus oryzae.

Methods in molecular biology (Clifton, N.J.)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 6, 2026

Autonomously Bioluminescent Mammalian Cells for Continuous and Real-time Monitoring of Cytotoxicity

Autonomously Bioluminescent Mammalian Cells for Continuous and Real-time Monitoring of Cytotoxicity

Published on: October 28, 2013

Cell-based bioluminescence screening assays.

Romain J Amante¹, Christian E Badr

¹Experimental Therapeutics and Molecular Imaging Laboratory, Neuroscience Center, Department of Neurology, Massachusetts General Hospital, Boston, MA, USA.

Methods in Molecular Biology (Clifton, N.J.)

|October 30, 2013

Summary

This summary is machine-generated.

This study introduces a new bioluminescence assay for high-throughput drug screening. The assay rapidly identifies potential anticancer molecules by monitoring cancer cell viability, offering a cost-effective discovery method.

More Related Videos

Bioluminescent Bacterial Imaging In Vivo

Bioluminescent Bacterial Imaging In Vivo

Published on: November 4, 2012

Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters

Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters

Published on: September 27, 2012

Related Experiment Videos

Last Updated: May 6, 2026

Autonomously Bioluminescent Mammalian Cells for Continuous and Real-time Monitoring of Cytotoxicity

Autonomously Bioluminescent Mammalian Cells for Continuous and Real-time Monitoring of Cytotoxicity

Published on: October 28, 2013

Bioluminescent Bacterial Imaging In Vivo

Bioluminescent Bacterial Imaging In Vivo

Published on: November 4, 2012

Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters

Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters

Published on: September 27, 2012

Area of Science:

Biomedical research
Drug discovery and development
Oncology

Background:

Drug screening is crucial for identifying new therapeutics.
Oncology relies heavily on effective drug discovery techniques.
High-throughput screening (HTS) accelerates the identification of potential drug candidates.

Purpose of the Study:

To describe a novel functional screening assay for drug discovery.
To enable high-throughput monitoring of cancer cell viability.
To facilitate the rapid and cost-effective identification of anticancer molecules.

Main Methods:

Development of a bioluminescence-based assay.
Utilizing secreted luciferase for cell viability detection.
Implementing a high-throughput format for screening large compound libraries.

Main Results:

The assay successfully monitors cancer cell viability.
It allows for the screening of thousands of compounds.
Potential anticancer molecules were identified efficiently.

Conclusions:

The described assay is a rapid, facile, and cost-effective method for drug screening.
This technique is valuable for oncology drug discovery.
The bioluminescence assay supports high-throughput identification of novel anticancer agents.