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Related Experiment Video

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The human oligodendrocyte proteome.

Keiko Iwata1, Cecilia C Café-Mendes, Andrea Schmitt

  • 1Department of Psychiatry and Psychotherapy, Ludwig Maximilians University of Munich (LMU), Munich, Germany; Research Center for Child Mental Development, University of Fukui, Japan; Department of Development of Functional Brain Activities, United Graduate School of Child Development, Osaka University, Kanazawa University, Hamamatsu University School of Medicine, Chiba University and University of Fukui, Fukui, Japan.

Proteomics
|October 30, 2013
PubMed
Summary

Researchers created a human oligodendroglial cell line proteome database, identifying 2290 proteins crucial for central nervous system myelination and brain disorders like multiple sclerosis.

Keywords:
BiomedicineBrainMO3.13Multiple sclerosisOligodendrocytesSchizophrenia

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Area of Science:

  • Neuroscience
  • Proteomics
  • Cell Biology

Background:

  • Oligodendrocytes (OLs) are vital for central nervous system (CNS) myelination.
  • Dysfunction of OLs is implicated in neurological disorders such as multiple sclerosis and schizophrenia.
  • A comprehensive proteomic understanding of OLs is essential for disease research.

Purpose of the Study:

  • To establish a reference proteome database for human oligodendrocytes using the MO3.13 cell line.
  • To identify proteins involved in OL function and their relevance to CNS disorders.
  • To provide data for the Chromosome-centric Human Proteome Project (C-HPP).

Main Methods:

  • Utilized the human oligodendroglial cell line MO3.13.
  • Employed SDS-PAGE for protein prefractionation.
  • Performed nanoflow liquid chromatography-mass spectrometry (LC-MS) analysis after in-gel digestion.

Main Results:

  • Identified approximately 11,600 unique peptides, resulting in 2290 proteins after stringent filtering.
  • Characterized proteins involved in nine distinct biological processes and various molecular functions.
  • Detected OL-specific proteins like myelin basic protein (MBP) and 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNP), alongside disease-associated proteins.

Conclusions:

  • The MO3.13 proteome database serves as a valuable resource for understanding OL biology.
  • This proteomic data aids in the study of demyelinating and oligodendroglial disorders.
  • The dataset contributes to the C-HPP initiative by classifying proteins by chromosomal origin.