Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

2.9K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.9K
Physiological Control of Respiration01:23

Physiological Control of Respiration

5.1K
Introduction
Breathing, a seemingly passive process, is regulated by the respiratory center in the brainstem. This center coordinates the involuntary control of respirations, which means it occurs without conscious effort, ensuring a smooth and uninterrupted pattern.
Regulation of Ventilation
The body maintains ventilation by monitoring levels of carbon dioxide (CO2), oxygen (O2), and hydrogen ion concentration (pH) in the arterial blood. Among these factors, the level of CO2 plays a crucial...
5.1K
Inhalational Anesthetics: Overview01:20

Inhalational Anesthetics: Overview

1.8K
Inhalation anesthetics are drugs that induce general anesthesia upon inhalation. They work by increasing the sensitivity of GABAA receptors or inhibiting NMDA receptors, leading to a decrease in central nervous system activity. The depth of anesthesia can be rapidly adjusted by changing the concentration of the inhaled gas. Some common examples of inhalational anesthetics include volatile liquids like isoflurane, desflurane, sevoflurane and gases like xenon and nitrous oxide. Isoflurane, a...
1.8K
Chemotherapy-Induced Nausea and Vomiting: Cannabinoids01:21

Chemotherapy-Induced Nausea and Vomiting: Cannabinoids

1.1K
Tetrahydrocannabinol (THC) is a phytocannabinoid that primarily interacts with the CB1 receptor, a type of G protein-coupled receptor (GPCR) predominantly in and around the chemoreceptor trigger zone (CTZ) and emetic center. THC also blocks the serotonin receptor activity in the dorsal vagal complex (DVC) by inhibiting serotonin release. THC exerts its anti-emetic effects through these interactions, which are beneficial for patients undergoing chemotherapy.
Two synthetic agonists of THC,...
1.1K
Analgesia and Pain Management01:25

Analgesia and Pain Management

3.4K
Pain is critical to various clinical pathologies, provoking an urgent need for effective management. Pain, whether acute or chronic, is a complex neurochemical process. Its alleviation depends on the type, with nonopioid analgesics effective for mild to moderate pain, such as musculoskeletal or inflammatory pain, while neuropathic pain responds best to anticonvulsants, tricyclic antidepressants, or serotonin/norepinephrine reuptake inhibitors. For severe acute or chronic pain, opioids may be...
3.4K
Chemical Factors Affecting Respiration Centers01:31

Chemical Factors Affecting Respiration Centers

3.0K
Chemical factors such as changing CO2, O2, and H+ levels in arterial blood play a critical role in influencing respiration depth and rates. These variations are detected by chemoreceptors—specialized sensors located in two primary body areas. Central chemoreceptors are found throughout the brain stem, including the ventrolateral medulla, while peripheral chemoreceptors are located in the aortic arch and carotid arteries.
CO2 has a potent influence on respiration and is strictly regulated....
3.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Influence of surface characteristics on the in vitro stability and cell uptake of nanoliposomes for brain delivery.

Beilstein journal of nanotechnology·2026
Same author

Oral Treatment of Obesity by GLP-1 and Its Analogs.

Pharmaceutics·2025
Same author

Optimizing microRNA delivery via albumin-decorated nanostructured lipid carriers.

International journal of pharmaceutics: X·2025
Same author

Novel development of lipid-based formulations: Improved wettability and homogeneous API solid dispersion visualised via near-infrared hyperspectral imaging.

European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V·2025
Same author

Acute high-fat high-sugar diet rapidly increases blood-brain barrier permeability in mice.

The journal of nutrition, health & aging..·2025
Same author

Fatty-acid amide hydrolase inhibition mitigates Alzheimer's disease progression in mouse models of amyloidosis.

The FEBS journal·2025

Related Experiment Video

Updated: May 6, 2026

Videomorphometric Analysis of Hypoxic Pulmonary Vasoconstriction of Intra-pulmonary Arteries Using Murine Precision Cut Lung Slices
13:32

Videomorphometric Analysis of Hypoxic Pulmonary Vasoconstriction of Intra-pulmonary Arteries Using Murine Precision Cut Lung Slices

Published on: January 14, 2014

12.6K

Endocannabinoid anandamide mediates hypoxic pulmonary vasoconstriction.

Daniela Wenzel1, Michaela Matthey, Laura Bindila

  • 1Institutes of Physiology I and Molecular Psychiatry, Life and Brain Center, University of Bonn, 53127 Bonn, Germany.

Proceedings of the National Academy of Sciences of the United States of America
|October 30, 2013
PubMed
Summary

The endocannabinoid anandamide (AEA) mediates hypoxic pulmonary vasoconstriction (HPV) through fatty acid amide hydrolase (FAAH). Inhibiting FAAH protects against hypoxia-induced pulmonary hypertension and vascular remodeling.

Keywords:
cannabinoidpulmonary vascular tone

More Related Videos

A Method of Nodose Ganglia Injection in Sprague-Dawley Rat
09:28

A Method of Nodose Ganglia Injection in Sprague-Dawley Rat

Published on: November 25, 2014

18.9K
Evaluation of Cerebral Blood Flow Autoregulation in the Rat Using Laser Doppler Flowmetry
07:12

Evaluation of Cerebral Blood Flow Autoregulation in the Rat Using Laser Doppler Flowmetry

Published on: January 19, 2020

8.9K

Related Experiment Videos

Last Updated: May 6, 2026

Videomorphometric Analysis of Hypoxic Pulmonary Vasoconstriction of Intra-pulmonary Arteries Using Murine Precision Cut Lung Slices
13:32

Videomorphometric Analysis of Hypoxic Pulmonary Vasoconstriction of Intra-pulmonary Arteries Using Murine Precision Cut Lung Slices

Published on: January 14, 2014

12.6K
A Method of Nodose Ganglia Injection in Sprague-Dawley Rat
09:28

A Method of Nodose Ganglia Injection in Sprague-Dawley Rat

Published on: November 25, 2014

18.9K
Evaluation of Cerebral Blood Flow Autoregulation in the Rat Using Laser Doppler Flowmetry
07:12

Evaluation of Cerebral Blood Flow Autoregulation in the Rat Using Laser Doppler Flowmetry

Published on: January 19, 2020

8.9K

Area of Science:

  • Pulmonary vascular research
  • Endocannabinoid system signaling
  • Cardiovascular physiology

Background:

  • Endocannabinoids regulate organ homeostasis, but their role in pulmonary vasculature is unclear.
  • Hypoxic pulmonary vasoconstriction (HPV) is a critical physiological response.
  • Pulmonary hypertension involves vascular remodeling and elevated pulmonary artery pressure.

Purpose of the Study:

  • To investigate the role of endocannabinoids, specifically anandamide (AEA), in HPV.
  • To determine the involvement of fatty acid amide hydrolase (FAAH) in AEA-mediated HPV.
  • To assess the therapeutic potential of targeting the AEA/FAAH pathway in pulmonary hypertension.

Main Methods:

  • Investigated AEA's effect on pulmonary arteries in vitro.
  • Utilized FAAH knockout (FAAH(-/-)) mice and pharmacological FAAH inhibition (URB597).
  • Measured AEA and arachidonic acid levels in hypoxic lungs using mass spectrometry.
  • Identified the cellular source of hypoxia-induced AEA.

Main Results:

  • AEA demonstrated a significant vasoconstrictive effect on pulmonary arteries.
  • FAAH(-/-) mice and URB597-treated mice exhibited reduced HPV.
  • Hypoxia increased AEA and arachidonic acid levels in wild-type mouse lungs.
  • Pulmonary vascular smooth muscle cells were identified as the source of hypoxia-induced AEA.
  • FAAH inhibition protected against hypoxia-induced pulmonary hypertension and vascular remodeling.

Conclusions:

  • The anandamide (AEA)/fatty acid amide hydrolase (FAAH) pathway is a key mediator of hypoxic pulmonary vasoconstriction (HPV).
  • Targeting the AEA/FAAH pathway offers a potential therapeutic strategy for pulmonary hypertension.