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Downregulation of PTEN expression in psoriatic lesions

Yadi Li1, Xiaohong Man, Liping You

  • 1Department of Dermatology, Xuanwu Hospital, Capital Medical University, Beijing, China; Department of Dermatology, Beijing Tongren Hospital, Capital Medical University, Beijing, China.

International Journal of Dermatology
|October 31, 2013
PubMed
Abstract

Insights

Reduced phosphatase and tensin homolog (PTEN) expression in psoriasis lesions may drive keratinocyte hyperproliferation by overactivating the PI3K/Akt pathway. This study investigated PTEN levels in psoriatic skin.

Area of Science:

  • Dermatology
  • Molecular Biology
  • Oncology

Background:

  • Tumor suppressor phosphatase and tensin homolog (PTEN) inhibits cell proliferation via the PI3K/Akt pathway.
  • PTEN loss-of-function is common in human tumors.
  • Akt activity is elevated in psoriatic lesions, but PTEN's role is unknown.

Purpose of the Study:

  • Investigate PTEN expression in psoriasis.
  • Determine the correlation between PTEN and the PI3K/Akt pathway in psoriasis.

Main Methods:

  • Quantitative RT-PCR to measure PTEN mRNA levels.
  • Western blotting and immunohistochemistry to assess PTEN protein expression.
  • Analysis of skin samples from 18 psoriasis patients and 19 healthy controls.

Main Results:

  • PTEN mRNA levels were significantly decreased in psoriatic lesions compared to normal skin.
  • PTEN protein expression was also lower in psoriatic lesions.
  • Reduced PTEN mRNA correlated with decreased PTEN protein levels.

Conclusions:

  • Downregulation of PTEN expression in psoriasis may contribute to PI3K/Akt pathway overactivation.
  • Reduced PTEN correlates with psoriatic keratinocyte hyperproliferation.

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