Related Experiment Video
Updated: May 6, 2026

Screening and Isolation of C-Glycoside-Cleaving Intestinal Bacteria
Published on: February 28, 2025
New phenanthrene glycosides from Dioscorea opposita
Ken Yu-Zhong Zheng1, Zhen-Xia Zhang, Wenliang Zhou
1a The School of Life Science, Sun Yat-Sen University , Guangzhou , 510275 , China.
Two new compounds, dioscopposide A and B from Dioscorea opposita, show potential in inhibiting nitric oxide production in immune cells.
Area of Science:
- Natural Product Chemistry
- Pharmacology
- Immunology
Background:
- Dioscorea opposita rhizomes are a source of bioactive compounds.
- Phenanthrene glycosides represent a class of natural products with diverse biological activities.
- Nitric oxide (NO) plays a crucial role in inflammatory processes.
Purpose of the Study:
- To isolate and characterize novel phenanthrene glycosides from Dioscorea opposita.
- To evaluate the inhibitory effects of these new compounds on nitric oxide production in macrophages.
Main Methods:
- Isolation of compounds using chromatographic techniques.
- Structure elucidation employing 1D and 2D NMR spectroscopy and Mass Spectrometry (MS).
- In vitro evaluation of anti-inflammatory activity using lipopolysaccharide (LPS)-stimulated murine macrophage RAW 264.7 cells.
Main Results:
- Two new phenanthrene glycosides, dioscopposide A and dioscopposide B, were successfully isolated and identified.
- Dioscopposide A exhibited an IC50 value of 5.8 μM against LPS-induced nitric oxide production.
- Dioscopposide B demonstrated an IC50 value of 7.2 μM in the same assay.
Conclusions:
- Dioscorea opposita is a valuable source for discovering new bioactive phenanthrene glycosides.
- Dioscopposide A and B possess significant inhibitory effects on nitric oxide production, suggesting potential anti-inflammatory properties.
More Related Videos
08:46Regioselective O-Glycosylation of Nucleosides via the Temporary 2',3'-Diol Protection by a Boronic Ester for the Synthesis of Disaccharide Nucleosides
Published on: July 26, 2018
07:59A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
Related Concept Videos
Preparation of Diols and Pinacol Rearrangement
The reaction begins with transferring a proton from the acid catalyst to one of the hydroxyl groups, producing an oxonium ion.
Aryldiazonium Salts to Azo Dyes: Diazo Coupling
Diels–Alder Reaction: Characteristics of Dienophiles
Characteristics of Dienophiles
Generally, the best dienophiles are alkenes containing electron-withdrawing substituents such as carbonyl, nitrile, and nitro groups. The feasibility of a Diels–Alder reaction...
Diels–Alder Reaction: Characteristics of Dienes
Characteristics of the diene
Conformation
The simplest example of a diene is 1,3-butadiene, an acyclic conjugated π system. At room temperature, the molecule exists as a mixture of s-cis and s-trans conformers by virtue of rotation around the carbon–carbon single bond. Although the s-trans isomer is...
Hydrolysis of Chlorobenzene to Phenol: Dow Process
Drugs Affecting GI Tract Motility: Opioids as Antidiarrheal Agents
Opioids, widely used antidiarrheal agents, mitigate diarrhea by slowing down...