Related Experiment Video
Updated: May 6, 2026

Frequency and Distribution of Crossovers in Caenorhabditis elegans Meiosis by SNP Genotyping using Real-time PCR
Published on: July 11, 2025
Heterozygosity in 2n gametes of potato evaluated by RFLP markers
A Barone1, C Gebhardt, L Frusciante
1Research Centre for Vegetable Breeding, Via Universitá 113, 80055, Portici, Italy.
Abstract:
The heterozygosity transmitted through 2n gametes in potato was evaluated by following the segregation of RFLP markers in tetraploid progeny from bilateral sexual polyploidization in a cross between two diploid (2x) interspecific hybrids which produce 2n SDR eggs or 2n FDR pollen. Out of 84 probe/enzyme combinations tested, 23 revealed polymorphism between the parents and were heterozygous in at least one of the parents. These probes characterized 13 loci distributed on five different chromosomes of the potato RFLP map. The heterozygosity transmitted through SDR and FDR gametes was estimated to be 31.8% and 71.4%, respectively. Two different indices (LH and RHI) were used to select plants showing a high level of heterozygosity in the tetraploid progeny. The recombination events and the centromere positions were estimated for chromosomes I, VI and VII, following the segregation ratios of SDR or FDR gametes produced by the parents. A different recombination rate was observed between the two interspecific hybrids.
More Related Videos
Related Concept Videos
Trihybrid Crosses
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal...
Dihybrid Crosses
Monohybrid Crosses
Law of Segregation
Chi-square Analysis
The chi-square test was developed by Pearson in 1990.
The first step of performing a Chi-square analysis is to establish a null hypothesis, which assumes that there is no real...
Law of Independent Assortment

