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Linear Amplification Mediated PCR – Localization of Genetic Elements and Characterization of Unknown Flanking DNA
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Detection of section-specific random amplified polymorphic DNA (RAPD) markers in Lilium.

M Yamagishi1

  • 1Research Institute of Agricultural Resources, Ishikawa Agricultural College, Ishikawa 921, Nonoichi-machi, Japan.

TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik
|October 31, 2013
PubMed
Summary
This summary is machine-generated.

Random amplified polymorphic DNA (RAPD) markers effectively identify Lilium species and hybrids. These DNA markers are specific to Lilium sections and hybrids, aiding in their precise identification.

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Area of Science:

  • Botany
  • Genetics
  • Molecular Biology

Background:

  • Accurate identification of Lilium species and hybrids is crucial for taxonomy and breeding.
  • Traditional methods may lack the precision required for distinguishing closely related Lilium taxa.
  • Molecular markers offer a powerful tool for genetic identification and characterization.

Purpose of the Study:

  • To investigate the utility of Random Amplified Polymorphic DNA (RAPD) markers for identifying Lilium species and inter-specific hybrids.
  • To identify section-specific DNA markers within the Lilium genus.
  • To assess the effectiveness of RAPD markers in distinguishing between different Lilium sections and their hybrids.

Main Methods:

  • Genomic DNA was amplified using polymerase chain reaction (PCR) with Random Amplified Polymorphic DNA (RAPD) primers.
  • An optimal annealing temperature of 54 °C was determined for the RAPD assay in Lilium.
  • Seventy-six primers were screened, and polymorphic DNA fragments were analyzed to identify unique markers.

Main Results:

  • 18 out of 76 primers (24%) successfully generated polymorphic DNA fragments in Lilium species and hybrids.
  • Six section-specific DNA markers were identified, enabling the differentiation of Lilium sections Sinomartagon, Leucolirion b, Leucolirion a, and Archelirion.
  • Seven inter-section hybrids displayed DNA bands characteristic of both parental sections, confirming the markers' utility.

Conclusions:

  • Random Amplified Polymorphic DNA (RAPD) markers are effective for the identification of Lilium species, cultivars, and inter-specific hybrids.
  • Section-specific DNA markers were discovered, facilitating the classification of Lilium taxa.
  • RAPD analysis provides a reliable method for determining the parental lineage of inter-section Lilium hybrids.