Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

LTR Retrotransposons03:08

LTR Retrotransposons

18.1K
LTR retrotransposons are class I transposable elements with long terminal repeats flanking an internal coding region. These elements are less abundant in mammals compared to other class I transposable elements. About 8 percent of human genomic DNA comprises LTR retrotransposons. Some of the common examples of LTR retrotransposons are Ty elements in yeast and Copia elements in Drosophila.
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
18.1K
Non-LTR Retrotransposons03:18

Non-LTR Retrotransposons

12.5K
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
12.5K
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

9.5K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
9.5K
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

3.1K
3.1K
DNA-only Transposons02:57

DNA-only Transposons

16.0K
DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
16.0K
Complementary DNA01:44

Complementary DNA

27.8K
Overview
27.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Homologies between nuclear and plastid DNA in spinach.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
Same author

Plastid-DNA levels in the different tissues of potato.

Planta·2013
Same author

Heterogeneity in cucumber ribosomal DNA.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
Same author

Structure of melon rDNA and nucleotide sequence of the 17-25S spacer region.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
Same author

A contiguous sequence in spinach nuclear DNA is homologous to three separated sequences in chloroplast DNA.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
Same author

Deletion mutation as a means of isolating avirulence genes in flax rust.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013

Related Experiment Video

Updated: May 6, 2026

Specific and Accurate Detection of the Citrus Greening Pathogen Candidatus liberibacter spp. Using Conventional PCR on Citrus Leaf Tissue Samples
09:23

Specific and Accurate Detection of the Citrus Greening Pathogen Candidatus liberibacter spp. Using Conventional PCR on Citrus Leaf Tissue Samples

Published on: June 29, 2018

7.2K

A hypervariable middle repetitive DNA sequence from citrus.

S J Orford1, N S Scott, J N Timmis

  • 1Department of Genetics, The University of Adelaide, 5005, Adelaide, South Australia.

TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik
|October 31, 2013
PubMed
Summary

Researchers identified a hypervariable DNA sequence in citrus plants. This sequence aids in distinguishing plant offspring and suggests that variations in these DNA markers are common in plants.

More Related Videos

Automating Citrus Budwood Processing for Downstream Pathogen Detection Through Instrument Engineering
11:30

Automating Citrus Budwood Processing for Downstream Pathogen Detection Through Instrument Engineering

Published on: April 21, 2023

1.4K
Determination of Self-Incompatibility and Inter-Incompatibility Relationships in Citrus Using Manual Pollination, Microscopy, and S-Genotype Analyses
07:12

Determination of Self-Incompatibility and Inter-Incompatibility Relationships in Citrus Using Manual Pollination, Microscopy, and S-Genotype Analyses

Published on: June 30, 2023

3.0K

Related Experiment Videos

Last Updated: May 6, 2026

Specific and Accurate Detection of the Citrus Greening Pathogen Candidatus liberibacter spp. Using Conventional PCR on Citrus Leaf Tissue Samples
09:23

Specific and Accurate Detection of the Citrus Greening Pathogen Candidatus liberibacter spp. Using Conventional PCR on Citrus Leaf Tissue Samples

Published on: June 29, 2018

7.2K
Automating Citrus Budwood Processing for Downstream Pathogen Detection Through Instrument Engineering
11:30

Automating Citrus Budwood Processing for Downstream Pathogen Detection Through Instrument Engineering

Published on: April 21, 2023

1.4K
Determination of Self-Incompatibility and Inter-Incompatibility Relationships in Citrus Using Manual Pollination, Microscopy, and S-Genotype Analyses
07:12

Determination of Self-Incompatibility and Inter-Incompatibility Relationships in Citrus Using Manual Pollination, Microscopy, and S-Genotype Analyses

Published on: June 30, 2023

3.0K

Area of Science:

  • Plant genetics
  • Molecular biology
  • Biotechnology

Background:

  • Hypervariable DNA sequences, including microsatellites and amplified fragment length polymorphisms (AFLP), are crucial for plant DNA typing.
  • Identifying and characterizing novel hypervariable DNA is essential for advancing plant genetics and breeding.

Purpose of the Study:

  • To isolate and characterize a novel hypervariable DNA sequence from citrus.
  • To assess the commercial application of this sequence for plant progeny analysis.
  • To investigate the occurrence of somatic variation in hypervariable markers.

Main Methods:

  • Isolation of a hypervariable length of middle repetitive DNA from citrus.
  • Development of a DNA fingerprinting probe from the isolated sequence.
  • Application of the probe for separating zygotic and nucellar progeny.
  • Analysis of somatic variants within an orange tree.

Main Results:

  • A novel hypervariable DNA sequence was isolated from citrus, lacking obvious hypervariable structures.
  • The developed fingerprinting probe demonstrated significant commercial value in distinguishing zygotic from nucellar offspring.
  • Evidence of a somatic variant within the sequence in an orange tree was observed.

Conclusions:

  • The isolated citrus DNA sequence is a valuable tool for plant DNA fingerprinting and progeny analysis.
  • Somatic variation in hypervariable markers appears to be a frequent occurrence in plants.
  • This research has implications for citrus breeding programs and genetic studies.