Biomolecule delivery to engineer the cellular microenvironment for regenerative medicine

Area of Science:

• Biomaterials Science
• Cell Biology
• Regenerative Medicine

Background:

• Cell fate is dictated by the cellular microenvironment, making controlled biomolecule delivery crucial for regenerative medicine.
• Effective tissue engineering and regenerative medicine necessitate bioengineered materials and cells for spatiotemporal control of biomolecule release and presentation.

Purpose of the Study:

• To review strategies for controlled biomolecule delivery in regenerative medicine.
• To discuss both intracellular and extracellular delivery methods, including gene transfer.
• To highlight therapeutic strategies and applications in tissue engineering.

Main Methods:

• Review of literature on biomolecule delivery systems.
• Discussion of soluble and insoluble biomolecule delivery.
• Exploration of gene transfer for intracellular delivery.
• Analysis of ex vivo and in vivo therapeutic approaches.
• Examination of combined delivery of biomolecules, scaffolds, and cells.

Main Results:

• Biomolecule delivery can be controlled from outside-in (soluble/insoluble) and inside-out (gene transfer).
• Ex vivo and in vivo strategies offer therapeutic potential.
• Combination therapies involving biomolecules, scaffolds, and cells show promise.
• Applications span bone tissue engineering and wound healing.

Conclusions:

• Precise control over biomolecule delivery is essential for advancing regenerative medicine.
• Diverse delivery strategies, including combination approaches, are being developed.
• Regenerative medicine holds significant potential for applications like bone regeneration and wound repair.