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Updated: May 6, 2026

Using Phylogenetic Analysis to Investigate Eukaryotic Gene Origin

Using Phylogenetic Analysis to Investigate Eukaryotic Gene Origin

Published on: August 14, 2018

Multiple sequence alignment with DIALIGN.

Burkhard Morgenstern¹

¹Abteilung für Bioinformatik (IMG), Universitat Göttingen, Göttingen, Germany.

Methods in Molecular Biology (Clifton, N.J.)

|October 31, 2013

Summary

This summary is machine-generated.

DIALIGN software aligns multiple sequences by combining global and local alignment features to find conserved functional regions. It uses local pairwise similarities and can incorporate external data for enhanced homology detection.

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Area of Science:

Bioinformatics
Computational Biology
Genomics

Background:

Multiple sequence alignment is crucial for identifying conserved regions and functional elements in biological sequences.
Discovering homology in sequences with limited overall similarity presents a significant challenge in bioinformatics.
Existing alignment tools may not effectively capture local similarities in otherwise divergent sequences.

Purpose of the Study:

To introduce DIALIGN, a software tool designed for multiple sequence alignment.
To highlight DIALIGN's capability in identifying conserved functional regions through local sequence similarities.
To present the latest enhancements in DIALIGN, including an anchoring option and PFAM database integration.

Main Methods:

DIALIGN combines global and local alignment strategies.
It composes multiple alignments by identifying local pairwise sequence similarities.
An anchoring option allows integration of external information and expert knowledge.
The latest version optionally utilizes PFAM database matches for weak homology detection.

Main Results:

DIALIGN successfully identifies conserved functional regions even in sequences with only local homologies.
The anchoring option enhances alignment accuracy by incorporating prior biological knowledge.
Integration with the PFAM database improves the detection of subtle or weak homologies.

Conclusions:

DIALIGN is an effective tool for multiple sequence alignment, particularly for discovering conserved functional regions in distantly related sequences.
The software's flexibility, including its anchoring option and PFAM integration, makes it valuable for various homology detection tasks.
DIALIGN is accessible via the Göttingen Bioinformatics Compute Server (GOBICS).