Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Diffraction methods for biological macromolecules. Least squares refinement.

R A Sparks

    Methods in Enzymology
    |January 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Space-group changes: a revision to a revision.

    Acta crystallographica. Section B, Structural science·2001
    Same author

    It could be more serious than you think!

    Genitourinary medicine·1995
    Same author

    Pancreatitis associated with aerosolised pentamidine.

    Genitourinary medicine·1995
    Same author

    Development of chronic renal disease in cats fed a commercial diet.

    Journal of the American Veterinary Medical Association·1993
    Same author

    Vulval neurofibromatosis masquerading as genital warts.

    International journal of STD & AIDS·1992
    Same author

    A 23-year-old white single insulin-dependent diabetic woman admitted as a gynaecological emergency with a 3-day history of painful vulval swelling and dysuria.

    International journal of STD & AIDS·1992
    Same journal

    1,2-Aminothiol-specific conjugation for dual-color fluorescent labeling via ultrafast TAMM conjugates.

    Methods in enzymology·2026
    Same journal

    Nitrone dipoles in bioorthogonal chemistry applications.

    Methods in enzymology·2026
    Same journal

    Bioorthogonal labeling of sialic acid isomers for detection of glycoconjugates by mass spectrometry imaging and microscopy.

    Methods in enzymology·2026
    Same journal

    Bioorthogonal photocatalytic proximity labeling for quantitative mapping of cell-cell interactions.

    Methods in enzymology·2026
    Same journal

    inCu-click: Enabling copper-catalyzed click chemistry inside living cells.

    Methods in enzymology·2026
    Same journal

    Site-specific antibody labeling via endo-S2 mediated Fc glycan remodeling.

    Methods in enzymology·2026
    See all related articles

    New crystallographic least-squares methods are more economical than older techniques, even for small molecules. These advancements offer efficient structure refinement for proteins and polynucleotides.

    Area of Science:

    • Crystallography
    • Computational Chemistry
    • Structural Biology

    Background:

    • Least-squares methods have been utilized in crystallography since the early 1960s.
    • The full-matrix least-squares method became popular due to advancements in computer technology.
    • Recent developments have focused on refining protein and polynucleotide structures.

    Purpose of the Study:

    • To evaluate the economic efficiency of different crystallographic least-squares methods.
    • To compare traditional methods with recent advancements in structure refinement.

    Main Methods:

    • Review of established crystallographic least-squares techniques.
    • Analysis of computational efficiency for various structure refinement approaches.
    • Focus on full-matrix least-squares and newer methods for macromolecular structures.

    Related Experiment Videos

    Main Results:

    • The full-matrix least-squares method, while popular, is computationally intensive.
    • Newer techniques, developed for protein and polynucleotide structure refinement, show improved efficiency.
    • These advanced methods are also more economical for small-molecule structures compared to the full-matrix approach.

    Conclusions:

    • Recent advancements in crystallographic least-squares calculations offer significant economic advantages.
    • These new techniques are applicable and beneficial for both small-molecule and macromolecular structure refinement.
    • The trend is towards more efficient and cost-effective computational methods in crystallography.