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Updated: May 6, 2026
Induction of Retinal Ischemia-Reperfusion Injury in a Mouse Eye Model
Published on: December 20, 2024
Evaluation of potential topical and systemic neuroprotective agents for ocular hypertension-induced retinal
Yi-Ing Chen1, Yih-Jing Lee, David A Wilkie
1Institute of Veterinary Clinical Sciences, School of Veterinary Medicine, National Taiwan University, No. 1, Section 4, Roosevelt Road, Taipei, 106, Taiwan.
Objective:
To evaluate for drugs with superior neuroprotective efficacy and investigate their underlying mechanisms related to antioxidation.
Procedures:
Brinzolamide (1%), timolol (0.5%), minocycline (22 mg/kg), lidocaine (1.5 mg/kg), and methylprednisolone (30 mg/kg) were administered to Sprague-Dawley (SD) rats. The retina was evaluated by electroretinography and histological analysis. The antioxidative capacity of drugs was evaluated to clarify the underlying mechanism. The oxidant/antioxidant profiles of plasma, red blood cells, and retina were analyzed by lipid peroxidation (malondialdehyde) and by measuring the activities of antioxidants. Proteomic analysis was used to investigate the possible protective mechanisms of the drug against ischemia-reperfusion injury.
Results:
The results suggested that timolol, methylprednisolone, and minocycline protected retinal function. Methylprednisolone and minocycline possessed good antioxidative activity. Brinzolamide and lidocaine preserved the structural integrity of the retina, but not retinal function.
Conclusion:
Methylprednisolone, minocycline, and timolol have potential acute or delayed benefit in retinal ischemia-reperfusion injury. Their neuroprotective actions depend at least partially on the ability to alleviate oxidative stress.
