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Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay
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Thrombin activity in normal vitreous liquid.

Thomas Bertelmann1, Walter Sekundo, Thomas Stief

  • 1aDepartment of Ophthalmology bDepartment of Laboratory Medicine, Philipps-University Marburg, Marburg, Germany cDepartment of Ophthalmology, Feldkirch Regional Hospital, Feldkirch, Austria.

Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis
|November 1, 2013
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Summary
This summary is machine-generated.

Physiologic thrombin activity is present in the vitreous of healthy eyes, measured for the first time. This finding may lead to new diagnostic tools for ophthalmology and understanding blood-retina barrier health.

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Area of Science:

  • Ophthalmology
  • Biochemistry
  • Retinal Physiology

Background:

  • The blood-retina barrier (BRB) maintains retinal homeostasis by controlling molecule passage from blood to the eye.
  • Thrombin, essential for hemostasis, may also play a role in preventing BRB breakdown.
  • The intraocular presence and activity of thrombin in healthy eyes remain unquantified.

Purpose of the Study:

  • To investigate and quantify intravitreal thrombin activity in eyes without BRB breakdown.
  • To establish a baseline for physiologic intraocular thrombin levels.
  • To explore potential diagnostic applications of intraocular thrombin activity.

Main Methods:

  • Vitreous samples (200 μl) were collected during pars plana vitrectomy from 16 participants.
  • Samples were stabilized and frozen for later analysis.
  • Chromogenic assays were used to quantify thrombin activity in thawed samples, with arginine protecting against non-specific substrate cleavage.

Main Results:

  • Intravitreal thrombin activity was detected in all 16 analyzed samples.
  • The mean thrombin activity was 1.5 ± 1.0 mIU/ml (range: 0.2-3.25 mIU/ml).
  • This represents the first successful quantification of physiologic intraocular thrombin activity.

Conclusions:

  • Physiologic levels of thrombin activity exist within the vitreous of healthy eyes.
  • Quantified intraocular thrombin activity could serve as a novel diagnostic parameter in ophthalmology.
  • Future research will compare these findings with thrombin activity in eyes with BRB breakdown.