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A 3D System for Culturing Human Articular Chondrocytes in Synovial Fluid

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Anti‑tuberculosis drugs decrease viability and stimulate the expression of chondrocyte marker genes in human nucleus

Tomasz P Lehmann¹, Maciej Głowacki, Ewa Misterska

¹Department of Biochemistry and Molecular Biology, Poznań University of Medical Sciences, Poznań 60‑781, Poland.

Molecular Medicine Reports

|November 1, 2013

Summary

First-line anti-tuberculosis drugs like isoniazid and rifampicin affect intervertebral disc cells. These drugs increase chondrocyte marker gene expression but can diminish cell viability, indicating potential side effects on disc health.

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Area of Science:

Biomedical Sciences
Cell Biology
Pharmacology

Background:

Tuberculosis (TB) affects bones, but the impact of anti-TB drugs on intervertebral disc (IVD) cells is unknown.
Nucleus pulposus (NP) cells are central to IVD health and function.
Osteoarticular TB (OATB) predominantly affects the spine, highlighting the need to understand drug effects on IVD cells.

Purpose of the Study:

To investigate the effects of first-line anti-tuberculosis drugs on human nucleus pulposus (NP) cells.
To determine the impact of isoniazid (INH), rifampicin (RIF), ethambutol (ETH), and pyrazinamide (PYR) on chondrocyte marker gene expression in IVD cells.
To assess the influence of these drugs on NP cell viability and glycosaminoglycan (GAG) production.

Main Methods:

Human NP cells were isolated from adolescent idiopathic scoliosis patients.
Cells were incubated with transforming growth factor-β1 (TGF-β1) and individual anti-TB drugs (INH, RIF, ETH, PYR).
Gene expression (ACAN, COL2A1, COL1A1, SOX9) and cell viability were analyzed after 24 and 192 hours, respectively. GAG concentrations were also measured.

Main Results:

Transforming growth factor-β1 significantly decreased ACAN mRNA levels.
Isoniazid increased COL2A1 and ACAN mRNA.
Rifampicin increased COL1A1 mRNA, and Pyrazinamide increased SOX9 mRNA.
Prolonged exposure to isoniazid and rifampicin reduced NP cell viability.
No significant changes in glycosaminoglycan concentrations were observed.

Conclusions:

First-line anti-tuberculosis drugs modulate chondrocyte marker gene expression in human IVD cells.
Isoniazid and rifampicin can negatively impact NP cell viability.
These findings suggest potential adverse effects of anti-TB drugs on intervertebral disc health beyond known systemic side effects.