The primary structure of a gene encoding yeast ribosomal protein L34

Sequence analysis revealed that a gene coding for yeast ribosomal protein L34 comprises an amino acid coding region of 339 nucleotides which is interrupted by an intron after the 19th codon. Like for other yeast ribosomal protein genes analyzed thus far a strong codon bias was observed. The flanking and intervening sequences of this gene encoding L34 show several elements that are conserved in a number of split ribosomal protein genes in yeast. Northern blot analysis using an intron-specific probe demonstrated that the sequenced gene copy coding for L34 is transcribed in vivo.