Related Experiment Video
Updated: May 6, 2026

mRNA Interactome Capture from Plant Protoplasts
Published on: July 28, 2017
Characterization of soybean chloroplast ribosomal RNA genes
G Singh1, G de Lanversin, N A Straus
1Department of Biology, University of Windsor, Windsor, N9B 3P4, Ontario.
Abstract:
Soybean chloroplast (cp) ribosomal RNA genes were located by hybridizing labeled homologous probes to filter bound cpDNA fragments. The soybean chloroplast chromosome, like those of most other plants, contains duplicate sets of ribosomal genes arranged in an inverted orientation. The inverted repeated regions are separated by two single copy regions of 21 and 116 kilobasepairs (kb). Cloned rDNA was mapped with numerous restriction endonucleases. This detailed study shows that the ribosomal genes are arranged in the sequence 5' -16S - Spacer - 23S - 3', and that both 16S and 23S rRNAs are transcribed from the same strand of DNA. Quantitative comparison of rDNA sequence divergence between soybean and tobacco indicates that the highest degree of divergence is in the spacer region, possibly due to an insertion/deletion.
Related Concept Videos
Ribosome Profiling
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Ribosomal RNA Synthesis
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosomal RNA Synthesis
Export of Mitochondrial and Chloroplast Genes
Protein Transport to the Inner Chloroplast Membrane
Ribosomes
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome...

