Improved procedure for the detection of activity of lipoxygenases on electropherograms

Several procedures for activity staining of lipoxygenases were compared. The best results were obtained with the o-dianisidine method of De Lumen and Kazeniac which had to be modified, however, with respect to the conditions of electrophoretic separation, treatment of the gel after separation, incubation conditions and staining solution, to achieve satisfactory detection of the lipoxygenases from wheat grains, pea seeds, soybeans and rabbit reticulocytes.